Actinomycetemcomitin:: a new bacteriocin produced by Aggregatibacter (Actinobacillus) actinomycetemcomitans
Actinomycetemcomitin:: a new bacteriocin produced by Aggregatibacter (Actinobacillus) actinomycetemcomitans
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DOI：
10.1007/s10295-007-0271-z
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发表时间：
2008-02-01
影响因子：
3.4
通讯作者：
Farias, Luiz de Macedo
中科院分区：
文献类型：
--
作者：
Lima, Francisca Lucia;Roque de Carvalho, Maria Auxiliadora;Farias, Luiz de Macedo
从牙周病患者分离出的聚集杆菌（Actinobacillus）放线菌 P7-20 菌株产生了一种细菌素（称为放线菌素），对厌氧消化链球菌 ATCC 27337 有活性。放线菌素在指数生长期和稳定生长期产生，在衰退生长期其量减少直至消失。通过硫酸铵沉淀（30-60% 饱和度）对其进行纯化，并进一步通过 FPLC（mono-Q 离子交换和 Phenyl Superose 疏水相互作用）和 HPLC（C-18 反相）进行纯化。在 pH 值低于 7.0 或高于 8.0 的条件下孵育、在 45 摄氏度下加热 30 分钟以及用胰蛋白酶、α-胰凝乳蛋白酶和木瓜蛋白酶等蛋白水解酶处理后，该细菌素会失去活性。 Actinomycetemcomitin 的分子量为 20.3 KDa，它是来自 A. actinomycetemcomitans 的一种新细菌素。
Aggregatibacter (Actinobacillus) actinomycetemcomitans P7-20 strain isolated from a periodontally diseased patient has produced a bacteriocin (named as actinomycetemcomitin) that is active against Peptostreptococcus anaerobius ATCC 27337. Actinomycetemcomitin was produced during exponential and stationary growth phases, and its amount decreased until it disappeared during the decline growth phase. It was purified by ammonium sulphate precipitation (30-60% saturation), and further by FPLC (mono-Q ionic exchange and Phenyl Superose hydrophobic interaction) and HPLC (C-18 reversed-phase). This bacteriocin loses its activity after incubation at a pH below 7.0 or above 8.0, following heating for 30 min at 45 degrees C, and after treatment with proteolytic enzymes such as trypsin, alpha-chymotrypsin, and papain. Actinomycetemcomitin has a molecular mass of 20.3 KDa and it represents a new bacteriocin from A. actinomycetemcomitans.