Anabolic signaling and protein deposition are enhanced by intermittent compared with continuous feeding in skeletal muscle of neonates
Anabolic signaling and protein deposition are enhanced by intermittent compared with continuous feeding in skeletal muscle of neonates
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DOI：
10.1152/ajpendo.00516.2011
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发表时间：
2012-03-01
影响因子：
5.1
通讯作者：
Davis, Teresa A.
中科院分区：
文献类型：
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作者：
El-Kadi, Samer W.;Suryawan, Agus;Davis, Teresa A.
El-Kadi SW, Suryawan A, Gazzaneo MC, Srivastava N, Orellana RA, Nguyen HV, Lobley GE, Davis TA。与连续喂养相比，间歇喂养增强了新生儿骨骼肌的合成代谢信号和蛋白质沉积。[J] .中国生物医学工程学报，2016,31(2):559 - 563。首次发表于2012年1月3日；doi: 10.1152 / ajpendo.00516.2011。口胃管喂养适用于进食能力受损的新生儿，可采用间歇给药或连续给药的方式。我们的目的是确定喂养方式是否影响肌肉蛋白质沉积，并确定其中的机制。对新生猪进行过夜禁食（FAS）或连续喂食等量食物（CON）或间歇喂食（7 × 4小时一餐），持续29小时。在第20至28小时之间的8小时内，猪注射[h -2(5)]苯丙氨酸和[h -2(2)]酪氨酸，并测量整个后腿的氨基酸（AA）净平衡。餐后INT组的胰岛素、支链AA、苯丙氨酸和酪氨酸动脉浓度以及全身苯丙氨酸和酪氨酸通量高于CON或FAS组。激活导致mRNA翻译起始的信号蛋白，包括真核起始因子（eIF）4E。与CON饲喂或FAS相比，INT可促进肌肉中eIF4G复合物的形成。除微管相关蛋白轻链3-II外，蛋白质降解信号蛋白不受饲喂方式的影响，其在FAS中含量最高。整个后躯的AA净去除量因INT而增加，而CON或FAS则没有增加，蛋白质沉积量因INT而增加。这是因为饲喂INT后蛋白质合成增加，但饲喂CON和FAS后蛋白质合成保持不变，而在任何饲料处理中蛋白质降解都没有变化。这些结果表明，间歇喂养比连续喂养更能促进新生儿肌肉蛋白质的增加，主要是通过增加蛋白质合成。
El-Kadi SW, Suryawan A, Gazzaneo MC, Srivastava N, Orellana RA, Nguyen HV, Lobley GE, Davis TA. Anabolic signaling and protein deposition are enhanced by intermittent compared with continuous feeding in skeletal muscle of neonates. Am J Physiol Endocrinol Metab 302: E674-E686, 2012. First published January 3, 2012; doi:10.1152/ajpendo.00516.2011.-Orogastric tube feeding is indicated for neonates with impaired ability to ingest and can be administered by intermittent bolus or continuous schedule. Our aim was to determine whether feeding modalities affect muscle protein deposition and to identify mechanisms involved. Neonatal pigs were overnight fasted (FAS) or fed the same amount of food continuously (CON) or intermittently (INT; 7 x 4 h meals) for 29 h. For 8 h, between hours 20 and 28, pigs were infused with [H-2(5)] phenylalanine and [H-2(2)] tyrosine, and amino acid (AA) net balances were measured across the hindquarters. Insulin, branched-chain AA, phenylalanine, and tyrosine arterial concentrations and whole body phenylalanine and tyrosine fluxes were greater for INT after the meal than for CON or FAS. The activation of signaling proteins leading to initiation of mRNA translation, including eukaryotic initiation factor (eIF)4E.eIF4G complex formation in muscle, was enhanced by INT compared with CON feeding or FAS. Signaling proteins of protein degradation were not affected by feeding modalities except for microtubule-associated protein light chain 3-II, which was highest in the FAS. Across the hindquarters, AA net removal increased for INT but not for CON or FAS, with protein deposition greater for INT. This was because protein synthesis increased following feeding for INT but remained unchanged for CON and FAS, whereas there was no change in protein degradation across any dietary treatment. These results suggest that muscle protein accretion in neonates is enhanced with intermittent bolus to a greater extent than continuous feeding, mainly by increased protein synthesis.