The ER network, peroxisomes and actin cytoskeleton exhibit dramatic alterations during somatic embryogenesis of cultured citrus cells
The ER network, peroxisomes and actin cytoskeleton exhibit dramatic alterations during somatic embryogenesis of cultured citrus cells
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内质网、过氧化物酶体和肌动蛋白细胞骨架在培养柑橘细胞的体细胞胚胎发生过程中表现出巨大的变化
DOI：
10.1007/s11240-021-02180-6
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发表时间：
2021-10-18
影响因子：
3
通讯作者：
Wang，Pengwei
中科院分区：
文献类型：
--
作者：
Wang，Kun;Gao，Erlin;Wang，Pengwei
体细胞胚胎发生技术是柑橘遗传改良的有效途径，克服了传统育种中的一些障碍。细胞内膜结构和细胞骨架的重塑对植物的生长和繁殖至关重要，但其在柑橘SE中的行为和功能尚未见报道。为了研究不同亚细胞结构在这一过程中的行为，我们用荧光蛋白标记了柑橘愈伤组织的多种内膜结构和细胞骨架网络。共聚焦显微镜研究表明，胚状体发育开始后，内质网的形态和过氧化物酶体的数量发生了显著变化。另一方面，肌动蛋白丝的排列也表现出显着的差异，在愈伤组织保持高胚发生潜力，相反，愈伤组织在形成胚是不稳定的。肌动蛋白解聚药物latrunculin B处理显著减少了体细胞胚的数量，表明肌动蛋白丝在调节柑橘SE中起重要作用。综上所述，我们的研究结果表明，细胞器的生物发生，形态建成和细胞骨架的动态重排是至关重要的，在柑橘SE。
Somatic embryogenesis (SE) is an effective approach for citrus genetic improvement, overcoming some of the obstacles presented in conventional breeding. It is known that the remodeling of endomembrane structures and cytoskeleton are essential for plant growth and reproduction, but their behaviors and functions in citrus SE has not been studied. In order to investigate the behaviors of various subcellular structures during this process, we used fluorescent proteins to label multiple endomembrane structures and cytoskeletal network in citrus callus. Confocal microscopy studies indicated that the morphology of endoplasmic reticulum and the numbers of peroxisomes changed significantly after the initiation of embryogenesis. On the other hand, the arrangement of actin filaments also exhibits significant differences in callus maintaining high embryogenic potential, in contrast to callus that are recalcitrant in forming embryos. The treatment of actin depolymerizing drug latrunculin B significantly reduced the number of somatic embryos, indicating that actin filaments play an important role in regulating SE in citrus. In summary, our results suggested that organelle biogenesis, morphogenesis and the dynamic rearrangement of cytoskeleton are crucial in SE of citrus.