Promising Cell-Based Therapy for Bone Regeneration Using Stem Cells From Deciduous Teeth, Dental Pulp, and Bone Marrow
Promising Cell-Based Therapy for Bone Regeneration Using Stem Cells From Deciduous Teeth, Dental Pulp, and Bone Marrow
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DOI：
10.3727/096368910x539128
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Nagasaka, Tetsuro
中科院分区：
文献类型：
--
作者：
Yamada, Yoichi;Ito, Kenji;Nagasaka, Tetsuro
我们试图再生骨在一个显着的骨缺损与各种干细胞从乳牙，提取幼犬，并将它们移植到父母犬下颌骨作为同种异体移植物，父母牙髓，和骨髓的组织工程和再生医学技术，使用血小板丰富的血浆作为自体支架和信号分子。最初，从儿童和父母杂交犬下颌骨区域和骨髓（犬间充质干细胞; cMSC）和父母牙齿（犬牙髓干细胞; cDPSC）中提取牙齿，并从乳牙中提取干细胞（小狗乳牙干细胞; pDTSC）。4周后，用环锯棒在下颌骨两侧制备骨缺损。将移植物材料植入这些缺损中：1）对照（仅缺损），2）富血小板血浆（PRP），3）cMSC/PRP，4）cDPSC/PRP，和5）pDTSC/PRP，以研究干细胞的作用。在第2、4和8周时，通过组织学和组织形态计量学分析对缺损处新形成的骨进行评价。根据组织学观察，与对照组（仅缺损）和PRP组相比，cMSCs/PRP、cDPSCs/PRP和pDTSCs/PRP组分别在4周和8周时具有良好形成的成熟骨和新生血管，并且cMSCs/PRP、cDPSCs/PRP和pDTSCs/PRP标本中的矿化组织在8周时为骨钙素阳性。组织学上，8周时新形成的骨面积分别为19.0 +/- 2.9%（对照）、19.7 +/- 6.0%（PRP）、52.8 +/- 3.5%（cMSC/PRP）、61.6 +/- 1.3%（cDPSC/PRP）和54.7 +/- 2.2%（pDTSC/PRP）。cMSC、cDPSC、pDTSC/PRP以及对照组和PRP组之间存在显著差异。这些结果表明，来自乳牙，牙髓和骨髓的干细胞与PRP具有形成骨的能力，并且与DTSC的骨形成可能具有在儿童和父母之间产生移植物的潜力。本研究为干细胞治疗在骨科和口腔颌面部重建中的临床应用奠定了基础。
We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasma as an autologous scaffold and signal molecules. Initially, teeth were extracted from a child and parent hybrid canine mandible region and bone marrow (canine mesenchymal stem cells; cMSCs), and parent teeth (canine dental pulp stem cells; cDPSCs), and stem cells were extracted from deciduous teeth (puppy deciduous teeth stem cells; pDTSCs). After 4 weeks, bone defects were prepared on both sides of the mandible with a trephine bar. Graft materials were implanted into these defects: 1) control (defect only), 2) platelet-rich plasma (PRP), 3) cMSCs/PRP, 4) cDPSCs/PRP, and 5) pDTSCs/PRP to investigate the effect of stem cells. The newly formed bones were evaluated by histology and histomorphometric analysis in the defects at 2, 4, and 8 weeks. According to histological observations, the cMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization compared with the control (defect only) and PRP groups at 4 and 8 weeks, respectively, and the mineralized tissues in cMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP specimens were positive for osteocalcin at 8 weeks. Histometrically, newly formed bone areas were 19.0 +/- 2.9% (control), 19.7 +/- 6.0% (PRP), 52.8 +/- 3.5% (cMSCs/PRP), 61.6 +/- 1.3% (cDPSCs/PRP), and 54.7 +/- 2.2% (pDTSCs/PRP) at 8 weeks. There were significant differences between cMSCs, cDPSCs, pDTSCs/PRP, and control and PRP groups. These results demonstrate that stem cells from deciduous teeth, dental pulp, and bone marrow with PRP have the ability to form bone, and bone formation with DTSCs might have the potential to generate a graft between a child and parent. This preclinical study could pave the way for stem cell therapy in orthopedics and oral maxillofacial reconstruction for clinical application.