The combination of β-carotene and vitamins improve the pregnancy rate at first fixed-time artificial insemination in grazing beef cows
The combination of β-carotene and vitamins improve the pregnancy rate at first fixed-time artificial insemination in grazing beef cows
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DOI：
10.1016/j.livsci.2018.09.002
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发表时间：
2018-11-01
影响因子：
1.8
通讯作者：
Baruselli, Pietro Sampaio
中科院分区：
文献类型：
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作者：
de Gouvea, Vinfrius Nunes;Alcantra Colli, Marcos Henrrique;Baruselli, Pietro Sampaio
本试验旨在研究β-胡萝卜素和维生素（ADE和生物素）配合饲喂对放牧肉牛繁殖性能的影响。在随机区组设计中，共使用了430头哺乳经产Nellore奶牛（Bos indicus，初始体重= 417 +/- 58 kg）。在试验开始时（固定时间人工授精- FTAI前30天），根据产犊日期、身体状况评分（BCS）对动物进行分配，并根据血液β-胡萝卜素浓度进行分组。处理是：对照（矿物质补充剂：无β-胡萝卜素和维生素）和处理（500 mg β-胡萝卜素、70，000 IU维生素的组合。A，10，000 IU维生素。D3，500 mg Vit. E和10 mg生物素/牛/天进入矿物质补充剂）。使用基于雌二醇/孕酮（E2/P4）的方案使动物同步化。在FTAI后30天和繁殖季节结束后30天评估妊娠率。在第11天的FTAI同步化方案之前，没有观察到周期率[黄体（CL）的存在; P = 0.31]的差异。P4器械移除和FTAI之间发情期奶牛的百分比在第一次（P = 0.39）和第二次FTAI（P = 0.18）组间无差异。将β-胡萝卜素和维生素组合到矿物质补充剂中增加了首次FTAI的妊娠率：对照组：57.6%（125/217）vs.治疗组：66.7%（142/213; P = 0.04），但在第二次FTAI时未发生：对照组：55.4%（51/92）vs.治疗组：50.7%（36/71; P = 0.52）和自然交配：对照组：46.3%（19/41）vs.给药组：31.4%（11/35; P = 0.07）或在繁殖季节结束时，对照组：89.9%（195/217）与处理组：88.7%（189/213; P = 0.77）。在非妊娠奶牛中，第一次（P = 0.93）和第二次（P = 0.89）妊娠诊断时，CL的发生率在组间无差异。与对照组奶牛相比，给药组奶牛在第二次妊娠诊断时的胚胎胸宽更大（P = 0.003）。首次FTAI妊娠率（30 d）与FTAI方案第11 d的BCS（P = 0.05）、第2 d的最大卵泡（P = 0.01）和第11 d的血β-胡萝卜素浓度（P = 0.06）呈正相关。将β-胡萝卜素、维生素ADE和生物素结合到放牧肉牛的矿物质补充剂中可以提高第一次FTAI的受胎率，并可以成为优化繁殖性能的工具。
The aim of this experiment was to evaluate the effects of feeding the combination of beta-carotene and vitamins (ADE and biotin) on reproductive performance of grazing beef cows. A total of 430 suckled multiparous Nellore cows (Bos indicus, initial body weigh = 417 +/- 58 kg) were used in a randomized block design. At the beginning of the trial (30 days before fixed-time artificial insemination - FTAI) animals were assigned according to calving date, body condition score (BCS) and blocked according to blood beta-carotene concentration. Treatments were: Control (mineral supplement: no beta-carotene and vitamins) and Treated (combination of 500 mg beta-carotene, 70,000 IU Vit. A, 10,000 IU Vit. D3, 500 mg Vit. E and 10 mg biotin/cow/day into the mineral supplement). Animals were synchronized using estradiol/progesterone (E2/P4) based protocol. Pregnancy rate was evaluated 30 days after FTAI and 30 days after the end of breeding season. No difference in the cyclicity rate [presence of corpus luteum (CL); P = 0.31] before FTAI synchronization protocol on day 11 was observed. The percentage of cows in estrus between P4 device removal and FTAI did not differ between groups at first (P = 0.39) and second FTAI (P = 0.18). The combination of beta-carotene and vitamins into the mineral supplement increased the pregnancy rate at first FTAI: Control: 57.6% (125/217) vs. Treated: 66.7% (142/213; P = 0.04) but not at second FTAI: Control: 55.4% (51/92) vs. Treated: 50.7% (36/71; P = 0.52) and to the natural mating: Control: 46.3% (19/41) vs. Treated: 31.4% (11/35; P = 0.07) or at the end of breeding season Control: 89.9% (195/217) vs. Treated: 88.7% (189/213; P = 0.77). In the non-pregnant cows, the occurrence of CL did not differ between groups at first (P = 0.93) and second (P = 0.89) pregnancy diagnostics. Treated cows had greater embryo thoracic width at second pregnancy diagnostic compared to Control cows (P = 0.003). Positive correlations were observed between the pregnancy rate at first FTAI (day 30) and BCS at day 11 (P = 0.05), largest follicle at day 2 (P = 0.01), and blood beta-carotene concentration on day-11 (P = 0.06) of FTAI protocol. Including the combination of beta-carotene, vitamins ADE and biotin into mineral supplement for grazing beef cows increases the conception rate at first FTAI and can be a tool to optimize the reproductive performance.