Transcription profiling of the Neurospora crassa response to a group of synthetic (thio)xanthones and a natural acetophenone.
Transcription profiling of the Neurospora crassa response to a group of synthetic (thio)xanthones and a natural acetophenone.
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DOI：
10.1016/j.gdata.2015.02.001
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Videira A
中科院分区：
文献类型：
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作者：
Pedro Gonçalves A;Silva N;Oliveira C;Kowbel DJ;Glass NL;Kijjoa A;Palmeira A;Sousa E;Pinto M;Videira A
氧杂蒽酮是一类以二苯并-γ-吡喃酮核为特征的杂环化合物。分析它们在细胞中的作用模式，即揭示基因表达的改变，是重要的，因为这些化合物具有潜在的治疗应用。因此，我们研究了丝状真菌粗糙脉孢菌的转录反应，一组合成的（硫代）吨酮衍生物具有抗肿瘤活性，使用高通量RNA测序。ABC转运蛋白的诱导。Crassa，特别是ATRB和CDR 4，是用氧杂蒽酮处理的常见结果。此外，我们发现了一组基因抑制的所有测试（硫代）氧杂蒽酮衍生物，这是唤起基因下调氧化应激过程中。N.用分离自土壤真菌暹罗奈氏菌的苯乙酮处理的crassa与（硫代）氧杂蒽酮诱导的基因表达谱具有一些特征。两种（硫代）口山酮衍生物和N. Siamensis-derived acetophenone抑制N.粗鲁。我们的工作还提供了框架数据集，可能会导向未来的研究机制的一些组的氧杂蒽酮。
Xanthones are a class of heterocyclic compounds characterized by a dibenzo-γ-pyrone nucleus. Analysis of their mode of action in cells, namely uncovering alterations in gene expression, is important because these compounds have potential therapeutic applications. Thus, we studied the transcriptional response of the filamentous fungus Neurospora crassa to a group of synthetic (thio)xanthone derivatives with antitumor activity using high throughput RNA sequencing. The induction of ABC transporters in N. crassa, particularly atrb and cdr4, is a common consequence of the treatment with xanthones. In addition, we found a group of genes repressed by all of the tested (thio)xanthone derivatives, that are evocative of genes downregulated during oxidative stress. The transcriptional response of N. crassa treated with an acetophenone isolated from the soil fungus Neosartorya siamensis shares some features with the (thio)xanthone-elicited gene expression profiles. Two of the (thio)xanthone derivatives and the N. siamensis-derived acetophenone inhibited the growth of N. crassa. Our work also provides framework datasets that may orientate future studies on the mechanisms of action of some groups of xanthones.