Template-directed synthesis on the oligonucleotide d(C7-G-C7).
Template-directed synthesis on the oligonucleotide d(C7-G-C7).
复制标题
寡核苷酸 d(C7-G-C7) 的模板定向合成。
DOI：
10.1016/0022-2836(86)90482-1
复制
发表时间：
1986
影响因子：
5.6
通讯作者：
Orgel,LE
中科院分区：
文献类型：
--
作者：
Haertle,T;Orgel,LE
当脱氧核苷酸模板d(C7-G-C7)与活化的核苷酸2-MeImpG†和2-MeImpC一起温育时，形成一系列G直至七聚体的寡聚物和一系列组成GnC且n=3至13的共聚物。大于 7 的寡聚物 GnC 会被胰腺核糖核酸酶完全降解，从而确定它们在 (pG)ipC(pG)j 形式的序列内的 5'-C 和 3'-G 之间含有 3' 至 5' 核苷酸间键。正如预期，(pG)7-Cp 和 (pG)6-Cp 是主要的水解产物。对产物分布的详细分析表明，大部分寡聚产物属于 (pG)ipC(pG)jwithi< 7 类型。这表明产物合成不一定从模板的 3' 末端开始。讨论了这一发现对于分子复制起源的意义。
When the deoxynucleotide template d(C7-G-C7) is incubated with the activated nucleotides 2-MeImpG†and 2-MeImpC, a series of oligomers of G up to the sevenmer and a series of copolymers of composition GnC withn= 3 to 13 are formed. Oligomers GnC withn> 7 are completely degraded by pancreatic ribonuclease, establishing that they contain a 3′ to 5′ internucleotide bond between 5′-C and 3′-G within a sequence of the form (pG)ipC(pG)j. As expected, (pG)7-Cp and (pG)6-Cp are major hydrolysis products.Detailed analysis of the product distribution shows that a substantial fraction of the oligomeric products are of the type (pG)ipC(pG)jwithi< 7. This shows that product synthesis does not necessarily begin at the 3′ terminus of the template. The significance of this finding in terms of the origin of molecular replication is discussed.