Antihypertensive effect of the peptides derived from casein by an extracellular proteinase from Lactobacillus helveticus CP790.
Antihypertensive effect of the peptides derived from casein by an extracellular proteinase from Lactobacillus helveticus CP790.
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DOI：
10.3168/jds.s0022-0302(94)77026-0
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发表时间：
1994-04
影响因子：
3.5
通讯作者：
N. Yamamoto;A. Akino;T. Takano
中科院分区：
文献类型：
--
作者：
N. Yamamoto;A. Akino;T. Takano
研究了瑞士乳杆菌CP790酶解α-S_1-和β-酪蛋白的多肽对血管紧张素转换酶的抑制活性。此外，还观察了酪蛋白水解物对SHR自发性高血压大鼠的降压作用。α-S_1和β-酪蛋白水解物均抑制该酶。其中一些多肽具有酶抑制活性，其中一种来自β-酪蛋白的多肽对酶有很大的抑制作用；抑制50%酶活性所需的血管紧张素转换酶抑制剂的浓度为4微米。酪蛋白水解物对自发性高血压大鼠有明显的降压作用，口服剂量为15 mg/kg体重。含有约0.3%多肽的瑞士乳杆菌CP790发酵的牛奶对体重为5ml/kg(15 mg多肽/公斤)的自发性高血压大鼠也有降压活性，而用瑞士乳杆菌CP791发酵的牛奶则没有这种活性。结果表明，培养液中的蛋白酶从酪蛋白中释放出的多肽对SHR大鼠有降压作用。
Peptides derived from alpha s1- and beta-caseins by the Lactobacillus helveticus CP790 proteinase were investigated for their inhibitory activities against angiotensin I-converting enzyme. The antihypertensive effect of casein hydrolysates in strain SHR spontaneously hypertensive rats was also investigated. Both alpha s1- and beta-casein hydrolysates inhibited this enzyme. Some of these peptides showed enzyme inhibitory activity, and one of them from beta-casein inhibited the enzyme greatly; the concentration of an angiotensin I-converting enzyme inhibitor needed to inhibit 50% of the enzyme activity was 4 microM. The hydrolysate of casein demonstrated antihypertensive activity in spontaneously hypertensive rats at an orally administered dosage of 15 mg/kg of body weight. MILK fermented with L. helveticus CP790, containing about .3% peptides, also showed antihypertensive activity in SHR rats with 5 ml/kg of body weight (15 mg of peptide/kg); however, the milk fermented with L. helveticus CP791, a variant defective for proteinase activity, did not show this activity. Results suggested that the peptides liberated from casein by the proteinase in the culture medium showed antihypertensive effect in SHR rats.