Plumbagin-mediating GLUT1 suppresses the growth of human tongue squamous cell carcinoma
Plumbagin-mediating GLUT1 suppresses the growth of human tongue squamous cell carcinoma
复制标题
白花丹素介导的 GLUT1 抑制人舌鳞状细胞癌的生长
作者：
Na S;Zhang J;Zhou X;Tang A;Huang D;Xu Q;Xue D;Qiu J
目的探讨人舌鳞状细胞癌（TSCC）中GLUT1的临床和组织病理学特征，以及白丹素（PLB）介导的GLUT1在TSCC生长中的作用。对象和方法收集45例TSCC标本，分析GLUT1的表达和定位。体外研究了PLB介导GLUT1对人TSCC细胞株CAL27生长的抑制作用及机制。结果经免疫组化染色，GLUT1在人TSCC组织中均有表达。GLUT1表达与TSCC淋巴结转移及临床分期有显著相关性。与体内对照组相比，PLB在体外通过抑制PI3K/Akt通路下调GLUT1表达，降低细胞活力和集落形成，增加细胞凋亡，抑制肿瘤生长，下调GLUT1表达。结论PLB通过抑制PI3K/Akt/GLUT1通路抑制TSCC生长，为PLB治疗TSCC提供理论依据。
ObjectiveThe purpose of this study was to investigate the clinical and histopathological characteristics of GLUT1 in human tongue squamous cell carcinoma (TSCC) and the role of plumbagin (PLB)‐mediating GLUT1 in the growth of TSCC.Subjects and methodsForty‐five cases of TSCC samples were collected and the expression and location of GLUT1 was analyzed. The role and mechanism of PLB meditating GLUT1 in the inhibitory growth of human TSCC cell line CAL27 were investigated invitroandvivo.ResultsThe expression of GLUT1 was observed in all samples of human TSCC by immunohistochemical staining. GLUT1 expression was significantly correlated with lymph node metastasis and clinical stage in TSCC. PLB treatment decreased cell viability and colony formation, and increased cell apoptosis in association with the downregulation of GLUT1 via inhibiting PI3K/Akt pathway invitroand PLB suppressed tumor growth in correlation with downregulation of GLUT1, compared with control group in vivo.ConclusionsThe findings demonstrated a novel anti‐cancer mechanism of PLB, inhibitory TSCC growth via suppressing PI3K/Akt/GLUT1 pathway, which will supply a theoretical basis for PLB to treat TSCC.