Disease resistance signature of the leucine-rich repeat receptor-like kinase genes in four plant species
Disease resistance signature of the leucine-rich repeat receptor-like kinase genes in four plant species
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DOI：
10.1016/j.plantsci.2010.06.017
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发表时间：
2010-10
期刊：
影响因子：
5.2
通讯作者：
Ping Tang;Ying Zhang;Xiaoqin Sun;Dacheng Tian;Sihai Yang;Jing Ding
中科院分区：
文献类型：
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作者：
Ping Tang;Ying Zhang;Xiaoqin Sun;Dacheng Tian;Sihai Yang;Jing Ding
类受体激酶（RLK）基因可能是植物抗病基因的一个潜在库，防御反应已被证明是其生物学功能的一个主要类别。虽然全基因组范围内的RLKs鉴定已在各种植物物种中完成，但很少有人区分与植物抗病性相关的基因。为了在植物中发现更多的R基因候选者，我们在四个完全测序的基因组中探索了所有的富含亮氨酸重复序列（LRR）RLKs，拟南芥，水稻，白杨和葡萄藤。系统发育分析表明，这四个物种的LRR-RLKs主要可分为两个相反的组：谱系特异性扩展与直系同源物明确非扩展。约39.2%的总LRR-RLKs居住在16个主要分支属于扩展组，表现出类似的特征，就典型的功能R基因。这些基因中有很高比例（72%）位于串联重复中，表明不依赖谱系的扩增在它们的进化中起着至关重要的作用。结果表明，非扩展组基因主要在植物生长发育和基础防御反应中起作用。由高度可变的病原体效应物驱动的正选择和基因扩增应该是造成两组LRR-RLKs之间差异的两个主要因素。
Receptor-like kinase (RLK) genes may be a potential reservoir for plant disease resistance (R) genes as defense response has been proved to be a main category of their biological functions. Although genome-wide identification of RLKs has been accomplished in various plant species, little was done to distinguish the genes related to plant disease resistance. To discover more R gene candidates in plants, we explored all the leucine-rich repeat (LRR) RLKs in four fully sequenced genomes, Arabidopsis thaliana, rice, poplar and grapevine. Phylogenetic analysis showed that LRR-RLKs in these four species could be mainly divided into two opposite groups: lineage-specific Expanded vs. ortholog-unambiguous Nonexpanded. About 39.2% of the total LRR-RLKs residing in 16 major clades belonged to the Expanded group, exhibiting similar characteristics with respect to typical functional R genes. A high proportion (72%) of these genes was located in tandem duplications, indicating that lineage-independent expansion played an essential role in their evolution. Contrarily, genes in the Nonexpanded group tended to function in plant growth and development and basal defense responses. Positive selection driven by highly variable pathogen effectors and gene expansion should be two major factors making the differences between the two groups of LRR-RLKs.