A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster
A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster
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DOI：
10.1073/pnas.97.25.13772
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发表时间：
2000-12-05
影响因子：
11.1
通讯作者：
Hultmark, D
中科院分区：
文献类型：
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作者：
Werner, T;Liu, G;Hultmark, D
来自细菌细胞壁的肽聚糖引发昆虫和哺乳动物的免疫反应。肽聚糖识别蛋白PGRP已从蛾类和脊椎动物中克隆，并已显示参与肽聚糖介导的蚕蛾酚氧化酶原活化。在这里，我们报告果蝇表达12个PGRP基因，分布在3条主要染色体上的8个染色体位点。通过分析cDNA克隆和基因组数据库，我们将它们分为两类：PGRP-SA。SB 1、SB 2、SC 1A、SC 1B、SC2和SD，具有短转录物和短5 '-非翻译区;以及PCRP-LA、LB、LC、LD和LE，具有长转录物和长5'-非翻译区。预测的结构表明，第一组编码胞外蛋白和第二组，细胞内和跨膜蛋白。大多数PGRP基因在胚后各阶段均有表达。肽聚糖注射强烈诱导五个基因。不同PGRP基因的转录本存在于免疫活性器官如脂肪体、肠道和血细胞中。我们证明，至少PGRP-SA和SC 1B可以结合肽聚糖，并在免疫功能可能是这个家庭。
Peptidoglycans from bacterial cell walls trigger immune responses in insects and mammals. A peptidoglycan recognition protein, PGRP, has been cloned from moths as well as vertebrates and has been shown to participate in peptidoglycan-mediated activation of prophenoloxidase in the silk moth. Here we report that Drosophila expresses 12 PGRP genes, distributed in 8 chromosomal loci on the 3 major chromosomes. By analyzing cDNA clones and genomic databases, we grouped them into two classes: PGRP-SA. SB1, SB2, SC1A, SC1B, SC2, and SD, with short transcripts and short 5'-untranslated regions; and PCRP-LA, LB, LC, LD, and LE, with long transcripts and long 5'-untranslated regions. The predicted structures indicate that the first group encodes extracellular proteins and the second group, intracellular and membrane-spanning proteins. Most PGRP genes are expressed in all postembryonic stages. Peptidoglycan injections strongly induce five of the genes. Transcripts from the different PGRP genes were found in immune competent organs such as fat body, gut, and hemocytes. We demonstrate that at least PGRP-SA and SC1B can bind peptidoglycan, and a function in immunity is likely for this family.