Synthesis of interleukin-5 by activated eosinophils in patients with eosinophilic heart diseases.
Synthesis of interleukin-5 by activated eosinophils in patients with eosinophilic heart diseases.
复制标题
嗜酸性粒细胞性心脏病患者中活化的嗜酸性粒细胞合成白细胞介素 5。
作者：
P. Desreumaux;A. Janin;S. Dubucquoi;M. Copin;G. Torpier;A. Capron;M. Capron;L. Prin
嗜酸性粒细胞心内膜疾病是慢性嗜酸性粒细胞相关疾病的主要进化风险。嗜酸性粒细胞颗粒蛋白似乎参与心脏损伤，但导致嗜酸性粒细胞浸润和脱颗粒的机制尚不清楚。白细胞介素-5 （IL-5）最近被证明是由嗜酸性粒细胞产生的，并可能在嗜酸性粒细胞的化学吸引和脱颗粒中发挥作用。在4例严重心力衰竭的嗜酸性粒细胞疾病中，我们评估了嗜酸性粒细胞表型的比例以及嗜酸性粒细胞阳离子蛋白（ECP）和可溶性IL-2受体（sIL-2R）的血清水平，这是嗜酸性粒细胞高综合征疾病活动的标志。所有4例患者均表现出低密度嗜酸性粒细胞比例明显增加，血清ECP和sIL-2R水平升高。在所有4例患者中，在心脏组织中观察到嗜酸性粒细胞颗粒蛋白的细胞外沉积和嗜酸性粒细胞活化的特征。通过原位杂交和抗人IL-5单克隆抗体免疫染色，在心肌切片和血细胞中检测到嗜酸性粒细胞合成IL-5。60% ~ 90%的组织嗜酸性粒细胞表达IL-5 mRNA和IL-5蛋白。这些数据提示IL-5可由心肌组织损伤部位的嗜酸性粒细胞产生，并可能参与局部嗜酸性粒细胞活化。
Eosinophilic endomyocardial disease represents a major evolutive risk in chronic eosinophilia-associated disorders. Eosinophil granule proteins appear to be involved in cardiac injury, but the mechanisms leading to eosinophil infiltration and degranulation are not clear. Interleukin-5 (IL-5) has been recently shown to be produced by eosinophils and might play a role in both chemoattraction and degranulation of eosinophils. In four cases of eosinophilic diseases with severe cardiac failure, we evaluated the proportion of eosinophil phenotypes and the serum levels of eosinophil cationic protein (ECP) and soluble IL-2 receptor (sIL-2R), markers of disease activity in the hypereosinophilic syndromes. All four patients showed a markedly increased proportion of hypodense eosinophils with elevated serum ECP and sIL-2R levels. In all four patients, extracellular deposition of eosinophil granule proteins and features of eosinophil activation were observed in cardiac tissues. The synthesis of IL-5 by eosinophils was detected in myocardial sections and blood cells by in situ hybridization and by immunostaining with a monoclonal antibody against human IL-5. Sixty percent to 90% of tissue eosinophils expressed IL-5 mRNA and IL-5 protein. These data suggest that IL-5 can be produced by eosinophils at the sites of myocardial tissue damage and might participate in local eosinophil activation.
霍奇金病伴嗜酸性粒细胞增多的 Reed-Sternberg 细胞中白细胞介素 5 信使 RNA 的检测。
影响因子：
20.3
作者：
Samoszuk,M;Nansen,L
通讯作者：
Nansen,L