A novel absolute quantitative imaging strategy of iron, copper and zinc in brain tissues by Isotope Dilution Laser Ablation ICP-MS
A novel absolute quantitative imaging strategy of iron, copper and zinc in brain tissues by Isotope Dilution Laser Ablation ICP-MS
复制标题
利用同位素稀释激光烧蚀ICP-MS对脑组织中铁、铜和锌进行绝对定量成像的新策略
DOI：
10.1016/j.aca.2017.07.003
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发表时间：
2017-09-01
影响因子：
6.2
通讯作者：
Li, Jiao
中科院分区：
文献类型：
--
作者：
Feng, Liuxing;Wang, Jun;Li, Jiao
同位素稀释激光烧蚀ICP-MS（ID-LA-ICP-MS）由于其令人印象深刻的空间分辨率能力和精确的定量手段，是用于生物样品中痕量元素的原位定量成像的最有前途的工具之一。在组织切片的ID-LA-ICP-MS策略中，组织在整个样品制备过程中必须保持完整。因此，如何在组织切片上均匀分布富集的同位素加标物以及如何确认样品和加标物之间的同位素平衡是两个重要的挑战。本研究提出了一种基于ID-LA-ICP-MS的生物薄片定量成像新方法。还研究了激光烧蚀和同位素交换参数以获得最佳ID-LA-ICP-MS条件。使用制备的均匀内部标准品验证ID-LA-ICP-MS方法，结果与原液分析结果一致。在此基础上，采用改进的方法对阿尔茨海默病（AD）真实的小鼠脑组织中Fe、Cu、Zn进行了定量显像测定。通过将LA-ICP-MS数据与Micro-XRF获得的数据进行比较，对真实的样品的方法进行了评估。此外，还对AD小鼠脑内元素分布和免疫组化标记进行了比较分析。相似的分布模式表明，所提出的方法是潜在的研究生物标志物的异质性和元素分布的相关性，并可能有助于了解这种复杂的大脑机制在未来。(C)2017爱思唯尔B. V.保留所有权利。
Isotope Dilution Laser Ablation ICP-MS (ID-LA-ICP-MS), because of its impressive spatial resolution capacity and precise means for quantification, is one of the most promising tools for in-situ quantitative imaging of trace elements in biological samples. In the ID-LA-ICP-MS strategy for tissue section, the tissue must be maintained intact during the whole sample preparation process. Therefore, how to homogeneously distribute enriched isotope spike on tissue section and how to confirm isotope equilibration between sample and spike are two important challenges. In this study, we reported a novel quantitative imaging strategy for biological thin section based on ID-LA-ICP-MS. To distribute the enriched isotope spikes on tissue section homogeneously, a "border" was constructed to make spike droplet stay on the tissue for isotope exchange. Laser ablation and isotope exchange parameters were also investigated to obtain optimal ID-LA-ICP-MS conditions. The prepared homogeneous in-house standard was used to validate the ID-LA-ICP-MS approach and good agreement with the bulk analysis was achieved. On this basis, quantitative imaging of Fe, Cu and Zn in real mouse brain of Alzheimer's Disease (AD) were measured by the improved methodology. Assessment of the method for real sample was undertaken by comparison of the LA-ICP-MS data with that obtained by micro-XRF. Moreover, comparative analysis of elements distribution and immunohistochemical markers in AD mouse brain was also carried out. The similar distributional patterns demonstrated that the proposed methodology is potential to investigate the correlation of biomarker heterogeneity and elements distribution, and may be useful to understand such complex brain mechanisms in the future. (C) 2017 Elsevier B.V. All rights reserved.