Light Chains of Myosin
Light Chains of Myosin
复制标题
肌球蛋白轻链
作者：
A. Weeds
来自兔骨骼肌的肌球蛋白含有低分子量蛋白质（轻链）以及构成分子主体的两条大多肽链。已使用碱处理1 -4来分离轻链和重链，并已通过凝胶电泳和超离心对其进行表征。分子量仍有争议，报告为20，000道尔顿2和32，000道尔顿3，但轻链在凝胶电泳上至少有三条带，这些分子量测定是用混合物进行的2，3。
MYOSIN from rabbit skeletal muscle contains low molecular weight proteins (light chains) as well as the two large polypeptide chains which comprise the bulk of the molecule. Alkali treatment has been used1–4 to separate the light from the heavy chains, and they have been characterized by gel electrophoresis and in the ultracentrifuge. The molecular weights are still in dispute, being reported as 20,000 daltons2 and 32,000 daltons3, but the light chains give at least three bands on gel electrophoresis and these molecular weight determinations2,3 were made with the mixture.