MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145
MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145
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DOI：
10.1099/mic.0.2006/003145-0
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发表时间：
2007-05-01
期刊：
影响因子：
2.8
通讯作者：
Challis, Gregory L.
中科院分区：
文献类型：
--
作者：
Lautru, Sylvie;Oves-Costales, Daniel;Challis, Gregory L.
MbtH样蛋白是一个小蛋白家族，由许多(但不是全部)非核糖体多肽合成酶编码基因簇中的基因编码，这些基因簇指导多肽抗生素和铁载体的生物合成。到目前为止发表的研究还没有阐明MbtH样蛋白的功能，也没有澄清它们是否是代谢产物生物合成所必需的。研究表明，天蓝色链霉菌中编码MbtH类蛋白的两个基因(CDAX或cchK)中只有一个基因是合成铁载体Coelichelin多肽和钙依赖多肽抗生素(CDA)所必需的。CDAX和cchK基因在反式作用中可以相互补充，提示CDAX和CchK可以分别与体轮蛋白和CDA生物合成途径相互作用。对野生型天蓝色链霉菌和一个双cchK/CDAX替换突变体的转录分析表明，CchK和CDAX可能不参与腔隙蛋白和CDA生物合成基因簇的转录调控。
MbtH-like proteins are a family of small proteins encoded by genes found in many, but not all, non-ribosomal peptide synthetase-encoding gene clusters that direct the biosynthesis of peptide antibiotics and siderophores. Studies published to date have not elucidated the function of MbtH-like proteins, nor have they clarified whether they are required for metabolite biosynthesis. Here it is shown that only one of two genes (cdaX or cchK) encoding MbtH-like proteins in Streptomyces coelicolor is required for biosynthesis of the peptide siderophore coelichelin and the calcium-dependent peptide antibiotic (CDA). The cdaX and cchK genes can functionally complement each other in trans, suggesting that CdaX and CchK can cross-talk with the coelichelin and CDA biosynthetic pathways, respectively. Transcriptional analyses of wild-type S. coelicolor and a double cchK/cdaX replacement mutant indicate that CchK and CdaX may not be involved in transcriptional regulation of coelichelin and CDA biosynthetic gene clusters.