THE CONFORMATION OF CHICKEN, RAT AND HUMAN U1-A RNAS IN SOLUTION
THE CONFORMATION OF CHICKEN, RAT AND HUMAN U1-A RNAS IN SOLUTION
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DOI：
10.1093/nar/9.4.841
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
JACOB, M
中科院分区：
文献类型：
--
作者：
BRANLANT, C;KROL, A;JACOB, M
鸡，大鼠和人类U1A RNA在溶液中，进行了检查的二级结构，使用几种方法，包括水解各种核酸酶，杂交的DNA寡聚体和片段相互作用的分析。实验结果表明，三种U1A RNA具有相同的结构，在较宽的pH和离子条件下稳定。它们允许从一级结构数据构建的几种可能的模型中选择一种。该模型的特征在于4个发夹和两个单链区域，来自分子3′部分的两个发夹具有非常稳定的茎。另外，实验结果表明，与分子的5′半相反，分子的3′半具有紧凑的构象，可能是通过三级相互作用稳定的。U1A RNA的5′端是可接近的，没有碱基配对，因此它可以与其他RNA分子的区域进行碱基配对，例如，最近在剪接模型中提出的内含子末端。
Chicken, rat and human U1A RNAs in solution, were examined for secondary structure, using several methods including hydrolysis by various nucleases, hybridization to DNA oligomers and analysis of fragment interactions. The experimental results showed that the three U1A RNAs have the same structure, stable over a wide range of pH and ionic conditions. They allowed the selection of one out of several possible models constructed from the data of primary structure. This model is characterized by 4 hairpins and two single-stranded regions, the two hairpins from the 3′ part of the molecule hearing very stable stems. In addition, the experimental results showed that in contrast to the 5′ half of the molecule, the 3′ half has a compact conformation probably stabilized by tertiary interactions. The 5′ end of U1A RNA is accessible and free of base-pairing so that it might base-pair with regions of other RNA molecules, for instance, with the extremities of introns as has been recently proposed in a model of splicing.