Cell proliferation in the dentate gyrus of rat hippocampus is inhibited by soft diet feeding
Cell proliferation in the dentate gyrus of rat hippocampus is inhibited by soft diet feeding
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DOI：
10.1159/000088700
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发表时间：
2005-01-01
期刊：
影响因子：
3.5
通讯作者：
Toyoda, M
中科院分区：
文献类型：
--
作者：
Aoki, H;Kimoto, K;Toyoda, M
背景：最近人们普遍认为神经发生发生在海马齿状回，海马齿状回是啮齿动物和人类记忆和学习功能的重要区域。报告显示，海马体的神经发生受到某些因素的调节，例如暴露于丰富的环境、体力活动、衰老和压力。作为口腔环境因素之一的咀嚼任务的变化与海马神经元生成机制之间的关系尚不清楚。目的：我们检查了大鼠海马齿状回中细胞增殖是否因咀嚼不同食物质地的任务而变化。方法：将Wistar大鼠分为两组，一组喂固体饲料，称为硬饲料喂养组，另一组喂食与固体饲料成分相同的粉末饲料，连续3、7、16和24周。胸苷类似物 5-溴-2'-脱氧尿苷 (BrdU) 被用作海马齿状回和嗅球内细胞增殖的标记物。结果：本研究结果表明，7、16、24周龄大鼠注射BrdU后，海马齿状回BrdU阳性细胞总数随着年龄的增长而显着减少，且软食喂养组明显少于硬食喂养组。 3周龄和24周龄大鼠嗅球中未观察到软食和硬食之间BrdU阳性细胞表达的变化。结论：表明软饲料喂养可显着抑制大鼠海马齿状回的细胞增殖。
Background: Recently it has become well accepted that neurogenesis occurs in the dentate gyrus of the hippocampus, a region important to memory and learning function in rodents and humans. Reports show that neurogenesis in the hippocampus is regulated by certain factors, such as exposure to an enriched environment, physical activity, aging and stress. The relationship between the change in the task of chewing as one oral environmental factor, and the mechanisms of hippocampal neuron generation are unclear. Objective: We examined whether cell proliferation varies by chewing tasks with different food textures in the dentate gyrus of the rat hippocampus. Methods: Wistar rats were divided into two groups, one was fed a solid diet, known as the hard-diet feeding group, and the soft-diet feeding group, which was fed a powder diet containing the same components as the solid one for 3, 7, 16 and 24 weeks. Thymidine analog 5-bromo-2'-deoxyuridine (BrdU) was used as a marker of cell proliferation within the dentate gyrus of the hippocampus and olfactory bulb. Results: The results of this study indicated that the total number of BrdU-positive cells in the hippocampal dentate gyrus significantly decreased with aging and were significantly fewer in the soft-diet feeding group than in hard-diet feeding group in 7-, 16- and 24-week-old rats after the BrdU injection. The change of BrdU-positive cell expression between soft and hard diets in 3- and 24-week-old rats was not observed in the olfactory bulb. Conclusion: It has been suggested that cell proliferation in the dentate gyrus of the rat hippocampus is characteristically suppressed by soft diet feeding.