Sequence of two rat insulin-like growth factor I mRNAs differing within the 5' untranslated region.
Sequence of two rat insulin-like growth factor I mRNAs differing within the 5' untranslated region.
复制标题
两种大鼠胰岛素样生长因子 I mRNA 的序列在 5 非翻译区有所不同。
DOI：
10.1093/nar/15.17.7196
复制
发表时间：
1987
影响因子：
14.9
通讯作者：
Rotwein,P
中科院分区：
文献类型：
--
作者：
Shimatsu,A;Rotwein,P
通过与源自大鼠胰岛素样生长因子 I (IGF-I) 基因 (1) 的探针杂交，筛选由 lambda gttO 中的大鼠肝脏多聚腺苷酸化 RNA 构建的 cDNA 文库。通过限制性内切酶图谱对十个重组噬菌体进行了表征。对两种类型的代表性 cDNA 进行了测序 (2)。两个序列均编码 159 个氨基酸的相同 IGF-I 蛋白前体。大 NH2 末端信号肽与人前肽的 43/48 个残基匹配 (3)。该蛋白质的其余部分，包括 70 个氨基酸 IGF-I（下划线），在哺乳动物物种中也高度保守 (1, 4)。 COOH 末端 25 个氨基酸似乎是通过涉及外显子 4 和 5 的替代 RNA 加工衍生的，正如针对小鼠 IGF-IB 的描述 (4)。两种大鼠 IGF-IB cDNA 在 5' 非翻译区内存在 186 bp 插入/缺失的差异，其连接点符合共有剪接信号 (5)。 IGF-I mRNA 的生成涉及多种机制，包括替代性 RNA 加工和替代性聚腺苷酸化 (1)。
A cDNA library, constructed from rat liver polyadenylated RNA in lambda gttO, was screened by hybridization with probes derived from the rat insulin-like growth factor I (IGF-I) genc (1). Ten recombinant phage were characterized by restriction endonuclease mapping. Representative cDNAs of two types were sequenced (2). Both sequiences encode identical IGF-I protein precursors of 159 amino acids. The large NH2-terminal signal peptide matches the human prepeptidc at 43/48 residues (3). The remainder of the protein, including 70 amino acid IGF-I (underlined), is also highly conserved among mammalian species (1, 4). The COOH-terminal 25 amino acids appear to be derived via alternative RNA processing involving exons 4 and 5, as has been described for mouse IGF-IB (4). The two rat IGF-IB cDNAs differ within the 5'untranslated region by an insertion/deletion of 186 bp whose junctions confornm to consensus splice signals (5). Multiple nmechanisms, including alternative RNA processing and alternative polyadenylation (1), are involved in teie generationi of IGF-I mRNAs.