Isolation and identification of a novel microcystin-degrading bacterium from a biological sand filter
Isolation and identification of a novel microcystin-degrading bacterium from a biological sand filter
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DOI：
10.1016/j.watres.2007.06.057
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发表时间：
2007-12-01
期刊：
影响因子：
12.8
通讯作者：
Newcombe, Gayle
中科院分区：
文献类型：
--
作者：
Ho, Lionel;Hoefel, Daniel;Newcombe, Gayle
从生物砂滤器中分离出一株能降解两种微囊藻毒素类似物微囊藻毒素LR和微囊藻毒素LA（MclA和MCLA）的新细菌，该细菌能有效去除沃茨中的这两种毒素。基于16 S rRNA基因序列的系统发育分析，分离的生物体，LH 21，最有可能属于属Sphingopyxis和以前培养的物种与Sphinyopyxis witflariensis聚类。使用聚合酶链反应（PCR），分离株LH 21显示含有先前与鞘氨醇单胞菌属物种ACM-3962降解甲氨蝶呤相关的四个基因（mlrA、mlrB、mlrC和mlrD）中的每一个的同源物。分离株LH 21能够在环境相关条件下在分批实验中有效降解MclO和MCLA，在毒素重新暴露后5 h内观察到完全去除。(c)2007爱思唯尔有限公司保留所有权利。
A novel bacterium capable of degrading two microcystin analogues, microcystin-LR and -LA (MCLR and MCLA), was isolated from a biological sand filter which was previously shown to effectively remove these toxins from source waters. Based on phylogenetic analysis of the 16S rRNA gene sequence, the isolated organism, LH21, most likely belonged to the genus Sphingopyxis and of the previously cultured species clustered with Sphinyopyxis witflariensis. Using polymerase chain reaction (PCR), isolate LH21 was shown to contain homologues to each of the four genes, mlrA, mlrB, mlrC and mlrD previously associated with the degradation of MCLR by Sphingomonas sp. ACM-3962. Isolate LH21 was able to effectively degrade MCLR and MCLA in batch experiments under environmentally relevant conditions, with complete removal observed within 5 h after re-exposure of the toxins. (c) 2007 Elsevier Ltd. All rights reserved.