Human A beta-amyloid and amyloid precursor protein accumulates in rat brain cells after cultured human leptomeningeal fibroblast implants.
Human A beta-amyloid and amyloid precursor protein accumulates in rat brain cells after cultured human leptomeningeal fibroblast implants.
复制标题
培养人软脑膜成纤维细胞植入物后，人 A β-淀粉样蛋白和淀粉样蛋白前体蛋白在大鼠脑细胞中积聚。
DOI：
10.1016/s0006-8993(96)01175-4
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发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Blass,JP
中科院分区：
文献类型：
--
作者：
DeGiorgio,LA;Bernstein,JJ;Manuelidis,L;Blass,JP
Cultured human leptomeningeal fibroblasts grafted into rat frontal cortex were localized to the implant pocket and to adjacent host leptomeninges. Immunohistochemical studies using a panel of human-specific and domain-specific APP antibodies revealed that all grafted cells expressed both APP and A β in situ. Remarkably, these antibodies also labeled rat pial and ependymal cells as well as reactive astrocytes adjacent to vessels. In addition, apical projections and cell bodies of many cortical pyramidal neurons contained human-specific APP immunoreactive material. Groups of subcortical neurons, particularly those of the amygdala, hippocampal formation and suprachiasmatic nuclei, were similarly labeled. The presence of human APP in host brains was confirmed by immunoblotting. Birefringent Congo Red staining was observed in the cortical neuropil and in leptomeningeal vessels. These data indicate that grafted leptomeningeal fibroblasts hyperexpress APP and A β which can diffuse into parenchyma and be taken up by specific rat cells.
