Protective efficacy of DNA vaccines against duck hepatitis B virus infection
Protective efficacy of DNA vaccines against duck hepatitis B virus infection
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DOI：
10.1128/jvi.72.1.84-94.1998
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发表时间：
1998-01-01
影响因子：
5.4
通讯作者：
Burrell, CJ
中科院分区：
文献类型：
--
作者：
Triyatni, M;Jilbert, AR;Burrell, CJ
用鸭B肝炎病毒（DHBV）前S/S蛋白和S蛋白DNA疫苗对北京鸭进行免疫效果试验。将含有DHBV pre-S/S或S基因的质粒pcDNA I/pcDNA肌肉注射3次，间隔3周。所有预S/S和S免疫的鸭在第三次免疫后产生了具有相似滴度的总抗DHBs和特异性抗S抗体，分别达到1/10，000至1/50，000和1/2，500至1/4，000。然而，在病毒攻击后，发现两组之间的血液中病毒清除率和肝脏中病毒复制存在显著差异。在四只S接种鸭中的三只中，90%的接种物在两次接种之间被去除。
The efficacy of DNA vaccines encoding the duck hepatitis B virus (DHBV) pre-S/S and S proteins were tested in Pekin ducks. Plasmid pcDNA I/Amp DNA containing the DHBV pre-S/S or S genes was injected intramuscularly three times, at 3-week intervals. All pre-S/S and S-vaccinated ducks developed total anti-DHBs and specific anti-S antibodies with similar titers reaching 1/10,000 to 1/50,000 and 1/2,500 to 1/4,000, respectively, after the third vaccination. However, following virus challenge, significant differences in the rate of virus removal from the bloodstream and the presence of virus replication in the liver were found between the groups. In three of four S-vaccinated ducks, 90% of the inoculum was removed between