Control by nutrients of growth and cell cycle progression in budding yeast, analyzed by double-tag flow cytometry
Control by nutrients of growth and cell cycle progression in budding yeast, analyzed by double-tag flow cytometry
复制标题
DOI：
10.1128/jb.180.15.3864-3872.1998
复制
发表时间：
1998-08-01
影响因子：
3.2
通讯作者：
Porro, D
中科院分区：
文献类型：
--
作者：
Alberghina, L;Smeraldi, C;Porro, D
为了深入了解细胞环境，生长特性和细胞周期进程的相互关系，我们分析了单个酿酒酵母细胞对周围环境变化和操纵的动态反应。我们使用了一种新的流式细胞术的方法，允许在异步生长的S。酿酒酵母群体，标记属于不同细胞周期设定点的细胞的细胞年龄和细胞蛋白质含量。由于细胞蛋白质含量是细胞大小的良好估计，因此可以在细胞周期进展期间跟踪细胞大小增加的动力学。所获得的研究结果的分析表明，在营养转移（从乙醇到葡萄糖）和添加环磷酸腺苷（cAMP）后，两个重要的延迟诱导。在营养向上转移的时刻在开始阶段之前循环的预先存在的细胞延迟它们进入S期，而在开始阶段之后循环的细胞延迟它们退出循环。这两种延迟的组合效应允许先前存在于向上移位的细胞群体快速地适应新的生长条件。还确定了营养下降的影响。
To gain insight on the interrelationships of the cellular environment, the properties of growth, and cell cycle progression, we analyzed the dynamic reactions of individual Saccharomyces cerevisiae cells to changes and manipulations of their surroundings. We used a new flow cytometric approach which allows, in asynchronous growing S. cerevisiae populations, tagging of both the cell age and the cell protein content of cells belonging to the different cell cycle set points. Since the cell protein content is a good estimation of the cell size, it is possible to follow the kinetics of the cell size increase during cell cycle progression. The analysis of the findings obtained indicates that both during a nutritional shift-up (from ethanol to glucose) and following the addition of cyclic AMP (cAMP), two important delays are induced. The preexisting cells that at the moment of the nutritional shift-up were cycling before the Start phase delay their entrance into S phase, while cells that were cycling after Start are delayed in their exit from the cycle. The combined effects of the two delays allow the cellular population that preexisted the shift-up to quickly adjust to the new growth condition. The effects of a nutritional shift-down were also determined.