Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures
Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures
复制标题
DOI：
10.1093/jxb/erj149
复制
发表时间：
2006-04-01
影响因子：
6.9
通讯作者：
Slabas, AR
中科院分区：
文献类型：
--
作者：
Chivasa, S;Hamilton, JM;Slabas, AR
利用经典的遗传学方法发现植物病原体与寄主互作的基因进展缓慢。广泛的研究采用DNA微阵列技术，以确定病原体-宿主相互作用过程中的基因表达的全球变化，大大提高了发现的遗传成分调节植物防御反应的病原体攻击。在这项研究中，一个互补的方法被用来确定蛋白质丰度的变化过程中相互作用的拟南芥细胞培养物与病原体衍生的激发子。可溶性蛋白组分进行了分析，通过二维差异凝胶电泳和蛋白质差异表达的真菌诱导剂处理进行了鉴定，通过基质辅助激光解吸电离飞行时间质谱。激发子应答蛋白包括分子伴侣、氧化应激防御蛋白、线粒体蛋白和多种代谢途径的酶。这些发现，结合目前可用的微阵列数据，将形成一个过滤器的基础，以确定关键基因，其在病原体防御系统中的作用将需要使用基因敲除突变体的确认。
Slow progress has been made in discovering plant genes governing the interaction of plant pathogens and their hosts using classical genetic approaches. Extensive studies employing DNA microarray techniques to identify global changes in gene expression during pathogen-host interaction have greatly enhanced discovery of genetic components regulating the plant defence response to pathogen attack. In this study, a complementary approach was used to identify changes in protein abundance during interaction of Arabidopsis cell cultures with a pathogen-derived elicitor. The soluble protein fractions were analysed by two-dimensional difference gel electrophoresis and proteins differentially expressed in response to treatment with fungal elicitor were identified via matrix-assisted laser desorption ionization-time of flight mass spectrometry. Elicitor responsive proteins included molecular chaperones, oxidative stress defence proteins, mitochondrial proteins, and enzymes of a diverse number of metabolic pathways. The findings, in combination with currently available microarray data, will form the basis of a filter to identify pivotal genes whose role in pathogen defence systems will require confirmation using gene knockout mutants.