Compositionally different desmosomes in the various compartments of the human hair follicle
Compositionally different desmosomes in the various compartments of the human hair follicle
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DOI：
10.1007/s002580050254
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发表时间：
1998-09-01
期刊：
影响因子：
2.9
通讯作者：
Franke, WW
中科院分区：
文献类型：
--
作者：
Kurzen, H;Moll, I;Franke, WW
毛囊是皮肤的复杂器官，在形态和个体发生上与表皮具有连续性。我们用免疫组织化学方法检测了成人和胎儿头皮毛囊中桥粒钙粘蛋白和桥粒斑块蛋白的位置，并建立了毛囊的定位“图”。使用针对斑块蛋白桥粒蛋白I和II、血小板红蛋白和嗜血小板蛋白1的抗体，我们发现这些发生在大多数（如果不是全部的话）毛囊桥粒中，而嗜血小板蛋白2则不存在，除了外根鞘的基底细胞，在那里发现了弱反应性。相比之下，桥粒钙粘蛋白大多是差异合成的，显示出一个复杂的地图。在所有细胞类型中均检测到desmocollin Dsc3，而Dsc1仅在根外鞘伴细胞层和根内鞘中检测到，而Dsc2几乎是互斥存在的。在根外鞘基底细胞、基底下外鞘中央细胞层、基质细胞和毛细胞中均可见到粘蛋白Dsg2，与Dsg1和Dsg3不同的免疫阳性反应部分重叠。我们还确定了这些蛋白质在胎儿毛囊发育过程中的合成时间。本文讨论了桥粒分子组成的差异与个体细胞类型之间可能存在的功能差异的关系。
Hair follicles are complex organs of the skin, in morphological and ontogenic continuity with the epidermis. We have examined the location of desmosomal cadherins and desmosomal plaque proteins in the hair follicle of adult and fetal human scalp skin by immunohistochemistry and have established a localization "map" of the hair follicle. Using antibodies against the plaque proteins desmoplakin I and II, plakoglobin, and plakophilin 1, we have found that these occur in most, if not all hair follicle desmosomes, whereas plakophilin 2 was absent, except in the basal cells of the outer root sheath, where a weak reactivity was found. By contrast, the desmosomal cadherins were mostly differentially synthesized, displaying a complicated map. While desmocollin Dsc3 was detected in all cell types examined,Dsc1 was detected only in the outer root sheath companion cell layer and the inner root sheath, and Dsc2 showed practically a mutually exclusive presence. Desmoglein Dsg2 was observed in basal cells of the outer root sheath as well as in the central cell layers of the subinfundibular outer rood sheath, matrix cells and trichocytes, in partial overlap with the otherwise different immunopositive reactions of Dsg1 and Dsg3. We have also determined when these proteins are synthesized during fetal hair follicle development. The differential molecular composition of desmosomes is discussed in relation to possible functional differences between the individual cell types.