Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp nov.
Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp nov.
复制标题
DOI：
10.1099/00207713-52-6-2309
复制
发表时间：
2002-11-01
影响因子：
2.8
通讯作者：
Nakase, T
中科院分区：
文献类型：
--
作者：
Bai, FY;Zhao, JH;Nakase, T
从云南植物叶片中分离得到50余株球孢子分生酵母，经常规方法鉴定为玫瑰孢菌（Sporobolomyces roseus Kluyver & van Niel）。然而，内部转录间隔区（ITS）和26S rDNA D1/D2结构域序列的比较表明，这些菌株代表不止一个物种。采用玫瑰孢子菌（Sporidiobolus roseus）的同义种和近缘种Sporidiobolus parparoseus Fell & Tallman进行rDNA序列比较。boleticomyces Ramirez、pollacciomyces Verona & Ciferri、roseomyces var madurae Janke和Torulopsis somala Verona与rosesporbolomyces同源。该种的另一个同义种salmoneus Derx与marcillae Santa Maria Sporobolomyces被放在一个单独的分支中。副副孢子虫的两个同义种，山崎和藤井孢子虫（未名inval.）和日本孢子虫（lizuka & Goto .）代表两个不同的种。以菌株CBS 4215(T)为类型菌株，验证了孢子菌（Sporobolomyces carnicolor）的名称。其中5个云南菌株为新种，命名为phaffii Sporobolomyces sp. nov.(类型菌株CH 2.052(T) = AS 2.2137(T) = JCM 11491（T) = CBS 9129(T)）。该研究还表明，具有相似ITS序列的酵母菌，其D1/D2序列可能存在较大差异。
More than 50 ballistoconidium-forming yeast strains, isolated from plant leaves collected in Yunnan, China, were identified as Sporobolomyces roseus Kluyver & van Niel by conventional methods. However, comparison of the internal transcribed spacer (ITS) region and 26S rDNA D1/D2 domain sequences indicated that these strains represented more than one species. Type or authentic strains of the synonyms of Sporobolomyces roseus and the closely related species Sporidiobolus pararoseus Fell & Tallman were employed in the rDNA sequence comparison. Sporobolomyces boleticola Ramirez, Sporobolomyces pollaccii Verona & Ciferri, Sporobolomyces roseus var. madurae Janke and Torulopsis somala Verona were confirmed to be conspecific with Sporobolomyces roseus. Another synonym of this species, Sporobolomyces salmoneus Derx, was located together with Sporobolomyces marcillae Santa Maria in a separate clade. Two synonyms of Sporidiobolus pararoseus, Sporobolomyces carnicolor Yamasaki & Fujii (nom. inval.) and Sporobotomyces japonicus lizuka & Goto, were revealed to represent two distinct species. The name Sporobolomyces carnicolor is validated, with strain CBS 4215(T) as the type strain. A novel species represented by five of the selected Yunnan strains was confirmed, for which the name Sporobolomyces phaffii sp. nov. is proposed (type strain CH 2.052(T) = AS 2.2137(T) = JCM 11491(T) = CBS 9129(T)). This study also indicates that yeast species with similar ITS sequences may have quite different D1/D2 sequences.