MICROTUBULE-ASSOCIATED PROTEIN-1B - IDENTIFICATION OF A MAJOR COMPONENT OF THE NEURONAL CYTOSKELETON
MICROTUBULE-ASSOCIATED PROTEIN-1B - IDENTIFICATION OF A MAJOR COMPONENT OF THE NEURONAL CYTOSKELETON
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DOI：
10.1073/pnas.82.16.5404
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
VALLEE, RB
中科院分区：
文献类型：
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作者：
BLOOM, GS;LUCA, FC;VALLEE, RB
纯化的脑微管中的主要非微管蛋白是高分子量的蛋白质，传统上分为微管相关蛋白1和2（MAP 1和MAP 2）。MAP 1由多肽复合物和最高MW物质组成，用单克隆抗体表征。四个单克隆抗体提出了对免疫纯化MAP 1B的描述。所有的抗体都只与这种蛋白质反应。结合肽图谱，这些结果表明MAP 1B在结构上不同于其他MAP。MAP 1B的另一个独特性质是，它的大部分在微管聚合过程中保持可溶性，导致其在大脑中的丰度被极度低估。大鼠脑切片和培养的大鼠脑细胞的免疫荧光显微镜显示，与MAP 1A和MAP 2相比，MAP 1B在轴突和树突过程中特别突出。显然，MAP 1B是神经元细胞骨架的一个主要的，以前未描述的组成部分。
The major nontubulin proteins in purified brain microtubules are high MW species traditionally classified into 2 groups known as microtubule-associated proteins 1 and 2 (MAP 1 and MAP 2). MAP 1 consisted of a complex of polypeptides and the highest MW species.sbd.MAP IA.sbd.was characterized with the use of a monoclonal antibody. Four monoclonal antibodies raised against electrophoretically purified MAP 1B are described. All of the antibodies reacted exclusively with this protein. Together with peptide mapping, these results indicated that MAP 1B was structurally distinct from the other MAP. Another distinctive property of MAP 1B was that most of it remained soluble during microtubule polymerization, resulting in an extreme underestimate of its abundance in the brain. Immunofluorescence microscopy of rat brain sections and cultured rat brain cells indicated that compared to MAP 1A and MAP 2, MAP 1B was particularly priminent in axonal as well as dendritic processes. Apparently, MAP 1B is a major, previously undescribed component of the neuronal cytoskeleton.