Apoptosis and expression of caspases 3, 6 and 8 in malignant non-Hodgkin's lymphomas
Apoptosis and expression of caspases 3, 6 and 8 in malignant non-Hodgkin's lymphomas
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DOI：
10.1111/j.1699-0463.1999.tb01508.x
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发表时间：
1999-11-01
期刊：
影响因子：
2.8
通讯作者：
Pääkkö, P
中科院分区：
文献类型：
--
作者：
Soini, Y;Pääkkö, P
本研究检测了85例恶性非霍奇金淋巴瘤和4例增生性淋巴结中caspase3、6和8的免疫组织化学表达。观察细胞凋亡程度及bcl2、bax的免疫组织化学表达。Caspase 3阳性84/85(99%)，caspase 6阳性46/85(54%)，caspase 8阳性66/85(78%)。Caspase3呈弥漫性胞浆免疫反应，caspase6和caspase8抗体呈颗粒状和碎片状，有时也呈核免疫阳性。高度恶性非霍奇金淋巴瘤表达Caspase6和Caspase8的频率显著高于低度恶性淋巴瘤(p=0.016和p=0.0002)。高级别淋巴瘤中caspase-3免疫反应较强，但相关性未达统计学意义(p=0.14)。Caspase3与Caspase6(p=0.032)、Caspase3与Caspase8(p=0.042)的表达呈正相关，尤其是caspase6与caspase8之间的表达(p=0.042)。
In this study we investigated the immunohistochemical expression of caspases 3, 6 and 8 in 85 malignant non-Hodgkin's lymphomas and in 4 hyperplastic lymph nodes. The extent of apoptosis and the immunohistochemical expression of bcl-2 and bax was also studied. Caspase 3 immunoreactivity was seen in 84/85 (99%), caspase 6 in 46/85 (54%), and caspase 8 in 66/85 (78%) lymphomas. The immunoreactivity for caspase 3 was diffuse cytoplasmic while antibodies to caspase 6 and 8 showed granular and fragmented, sometimes also nuclear immunopositivity. High-grade non-Hodgkin's lymphomas expressed strong caspase 6 and 8 immunoreactivity significantly more often than low-grade lymphomas (p=0.016 and p=0.0002, respectively). Strong caspase 3 immunoreactivity was also seen more often in high-grade lymphomas, but the association did not reach statistical significance (p=0.14). There was a strong association between the expression of caspase 3 and 6 (p=0.032), caspase 3 and 8 (p=0.042), and especially between caspase 6 and 8 (p=