Zinc stress induces copper depletion in Acinetobacter baumannii.
Zinc stress induces copper depletion in Acinetobacter baumannii.
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DOI：
10.1186/s12866-017-0965-y
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发表时间：
2017-03-11
期刊：
影响因子：
4.2
通讯作者：
Eijkelkamp BA
中科院分区：
文献类型：
--
作者：
Hassan KA;Pederick VG;Elbourne LD;Paulsen IT;Paton JC;McDevitt CA;Eijkelkamp BA
第一行过渡金属离子锌和铜是许多生物体生存所必需的，尽管过量的这些离子与显着的毒性有关。在这里，我们研究了锌和铜胁迫对鲍曼不动杆菌，一种常见的条件致病菌的影响。我们表明，细胞外锌胁迫诱导铜特异性亏损表型在A。鲍曼不动杆菌ATCC 17978。补充铜不仅不能挽救这种表型，而且进一步加剧了铜的消耗。对A.鲍曼不动杆菌ATCC 17978基因组鉴定了13个推定的锌/铜抗性外排泵。转录分析表明，其中4个转运蛋白对锌胁迫有反应，5个转运蛋白对铜胁迫有反应，7个转运蛋白对锌铜复合胁迫有反应，从而揭示了锌诱导的铜饥饿可能的基础。鲍曼不动杆菌。此外，我们表明，锌和铜在氧化应激和膜组成的管理中起着至关重要的作用。鲍曼不动杆菌。此外，我们发现，锌和铜在巨噬细胞介导的杀死这种病原体中发挥不同的作用。总的来说，本研究支持靶向金属离子稳态机制作为针对多药耐药细菌病原体的有效抗菌策略。本文的在线版本（doi：10.1186/s12866-017-0965-y）包含补充材料，可供授权用户使用。
The first row transition metal ions zinc and copper are essential to the survival of many organisms, although in excess these ions are associated with significant toxicity. Here, we examined the impact of zinc and copper stress on Acinetobacter baumannii, a common opportunistic pathogen. We show that extracellular zinc stress induces a copper-specific depletion phenotype in A. baumannii ATCC 17978. Supplementation with copper not only fails to rescue this phenotype, but further exacerbates the copper depletion. Extensive analysis of the A. baumannii ATCC 17978 genome identified 13 putative zinc/copper resistance efflux pumps. Transcriptional analyses show that four of these transporters are responsive to zinc stress, five to copper stress and seven to the combination of zinc and copper stress, thereby revealing a likely foundation for the zinc-induced copper starvation in A. baumannii. In addition, we show that zinc and copper play crucial roles in management of oxidative stress and the membrane composition of A. baumannii. Further, we reveal that zinc and copper play distinct roles in macrophage-mediated killing of this pathogen. Collectively, this study supports the targeting of metal ion homeostatic mechanisms as an effective antimicrobial strategy against multi-drug resistant bacterial pathogens. The online version of this article (doi:10.1186/s12866-017-0965-y) contains supplementary material, which is available to authorized users.