Odontoblasts enhance the maturation of enamel crystals by secreting EMSP1 at the enamel dentin junction
Odontoblasts enhance the maturation of enamel crystals by secreting EMSP1 at the enamel dentin junction
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DOI：
10.1177/154405910208101003
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发表时间：
2002-10-01
影响因子：
7.6
通讯作者：
Oida, S
中科院分区：
文献类型：
--
作者：
Fukae, M;Tanabe, T;Oida, S
研究了未成熟牙釉质中主要的两种蛋白酶——珐琅质蛋白酶（enamelysin）和EMSP1的时间表达模式和活性分布。从发育中的猪门牙中分离出细胞外基质，分别由牙本质前期、牙本质和四个分泌阶段的牙釉质样品组成，包括牙釉质-牙本质交界处的高矿化牙釉质（HME），并通过酶谱法鉴定了它们的活性蛋白酶。软组织部分，包括牙釉质形成延伸部位（ESEF）、分泌期和成熟期成釉细胞和成牙细胞，通过组织学和RT-PCR对其表达的珐琅质蛋白酶和EMSP1进行了表征。一个重要的发现是，由成牙细胞表达的EMSP1集中在HME中，但在前牙本质或牙本质中未检测到。我们得出结论，成牙细胞通过其细胞过程将EMSP1沉积到最深处的牙釉质层，这促进了该层的硬化，并对EDJ的功能特性有重要贡献。
The temporal expression patterns and activity distributions of enamelysin and EMSP1, which are the major proteinases in immature enamel, were characterized. Extracellular matrix fractions from developing porcine incisors, individually comprised of predentin, dentin, and four secretory-stage enamel samples, including the highly mineralized enamel (HME) at the enamel-dentin junction (EDJ), were isolated, and their resident proteinases were identified by zymography. Soft-tissue fractions, which included cells from the extension site of enamel formation (ESEF), secretory- and maturation-stage ameloblasts, and odontoblasts were characterized histologically and by RT-PCR for their expression of enamelysin and EMSP1. A significant finding was that EMSP1, expressed by odontoblasts, concentrates in the HME, but is not detected in predentin or dentin. We conclude that odontoblasts deposit EMSP1 via their cell processes into the deepest enamel layer, which facilitates the hardening of this layer and contributes significantly to the functional properties of the EDJ.