Electroretinograms in Drosophila: A Robust and Genetically Accessible Electrophysiological System for the Undergraduate Laboratory
Electroretinograms in Drosophila: A Robust and Genetically Accessible Electrophysiological System for the Undergraduate Laboratory
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果蝇视网膜电图：用于本科实验室的强大且遗传可访问的电生理系统
DOI：
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Karl G. Johnson
中科院分区：
文献类型：
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作者：
I. Vilinsky;Karl G. Johnson
神经生理学实验室课程满足了神经科学和生物学本科课程基于探究的培训的关键需求。这些课程通常使用经典的电生理学准备来探索神经元功能的基本特征。然而，当前的神经科学研究还侧重于使用包括突变和转基因动物的模型系统来阐明神经元功能的分子和遗传机制。为了将神经生理学的实验室培训与现代分子遗传学联系起来，我们描述了一种基于果蝇视网膜电图的教学模型，果蝇是一种长期建立的基础神经科学研究模型系统。果蝇易于维护、经济，并且拥有数百种神经生理学相关的突变株和容易获得的遗传工具。果蝇视网膜电图（ERG）是一种简单易行的细胞外记录，记录果蝇眼中响应闪光的神经信号。信号是多方面的，响应对强度、持续时间和波长等刺激参数敏感，从而为学生形成丰富的分析来源。最重要的是，影响细胞内信号传导、突触传递或神经元功能关键组成部分的不同突变可以以特有的方式影响 ERG 波形。记录野生型和突变型 ERG 使学生能够直接检查遗传学、生化途径和电生理学之间的联系。该神经生理学实验室课程可以促进和增强对细胞和分子对神经生理学记录的贡献的理解。
Laboratory courses in neurophysiology fulfill a critical need for inquiry-based training in undergraduate programs in neuroscience and biology. These courses typically use classical electrophysiological preparations to explore the basic features of neuronal function. However, current neuroscience research also focuses on elucidating the molecular and genetic mechanisms of neuronal function, using model systems that include mutant and transgenic animals. To bridge laboratory training in neurophysiology with modern molecular genetics, we describe a teaching model based on electroretinography of the fruit fly Drosophila melanogaster, a long-established model system for basic neuroscience research. Drosophila are easily maintained, economical, and have hundreds of neurophysiologically relevant mutant strains and genetic tools readily available. The Drosophila electroretinogram (ERG) is a simple and accessible extracellular recording of a neural signal in the fly eye in response to flashes of light. The signal is multifaceted and the response is sensitive to stimulation parameters such as intensity, duration and wavelength, thus forming a rich source of analysis for students. Most importantly, different mutations affecting key components of intracellular signaling, synaptic transmission or neuronal function can affect the ERG waveform in characteristic ways. Recording wild type and mutant ERGs allows students to examine firsthand the connection between genetics, biochemical pathways, and electrophysiology. This neurophysiology laboratory course can facilitate and enhance an understanding of the cellular and molecular contributions to neurophysiological recordings.
影响因子：
2.1
作者：
Pulver, Stefan R.;Hornstein, Nicholas J.;Johnson, Bruce R.
通讯作者：
Johnson, Bruce R.
影响因子：
3.3
作者：
R. Stowers;T. Schwarz
通讯作者：
R. Stowers;T. Schwarz