Specificity of the retrograde axonal transport of nerve growth factor.
Specificity of the retrograde axonal transport of nerve growth factor.
复制标题
神经生长因子逆行轴突运输的特异性。
DOI：
10.1016/0006-8993(74)90818-x
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
H. Thoenen
中科院分区：
文献类型：
--
作者：
K. Stöckel;U. Paravicini;H. Thoenen
用~（125）I标记的神经生长因子（NGF）及其类似蛋白质（在生理pH下的分子量和电荷）注射到成年小鼠眼前房，研究了NGF逆行轴突转运的特异性。先前的研究表明，注射侧和非注射侧的上级颈神经节中放射性积聚之间的差异是逆行轴突运输的量度。在研究的所有蛋白质（NGF、细胞色素c、胰岛素、辣根过氧化物酶、卵清蛋白、牛白蛋白、铁蛋白）中，NGF是唯一在注射侧和非注射侧之间表现出统计学显著（2-3倍）差异的蛋白质。相对较小的化学变化，如NGF分子的色氨酸部分的氧化，导致逆行转运的显著减少，这进一步支持了NGF逆行转运的特异性。结论是，以NGF形式从肾上腺素能神经末梢向细胞体提供信息的系统是高度特异性的，并且这种特异性不依赖于分子的一般物理化学性质，例如分子的大小和生理pH下的电荷。然而，到目前为止，现有的信息不允许人们决定选择性是否存在于运输系统本身或运输系统的特殊性。神经末梢的摄取机制。
The specificity of the retrograde axonal transport of nerve growth factor (NGF) has been investigated by injecting125I-labelled NGF and similar proteins (both with respect to molecular weight and electrical charge at physiological pH) into the anterior eye chamber of adult mice. Previous studies have shown that the difference between the accumulation of radioactivity in the superior cervical ganglion of the injected and non-injected sides is a measure for retrograde axonal transport. Of all the proteins studied (NGF, cytochrome c, insulin, horseradish peroxidase, ovalbumin, bovine albumin, ferritin), NGF was the only which exhibited a statistically significant (2–3 fold) difference between injected and non-injected sides. The specificity of the retrograde transport of NGF was further supported by the finding that relatively small chemical changes, such as oxidation of the tryptophan moieties of the NGF molecule, resulted in a marked reduction of retrograde transport.It is concluded that the system for providing information to the cell body from the adrenergic nerve terminals in the form of NGF is highly specific, and that this specificity does not depend on general physico-chemical properties of the molecule such as size of the molecule and electrical charge at physiological pH. However, the information available so far does not allow one to decide as to whether the selectivity resides in the transport system itself or in the specificity of the uptake mechanism at the nerve terminals.