Effect of silicon on grain yield of rice under cadmium-stress
Effect of silicon on grain yield of rice under cadmium-stress
复制标题
镉胁迫下硅对水稻产量的影响
DOI：
10.1007/s11738-016-2177-8
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发表时间：
2016-06-01
影响因子：
2.6
通讯作者：
Lin, Wenxiong
中科院分区：
文献类型：
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作者：
Lin, Hongmei;Fang, Changxun;Lin, Wenxiong
许多出版物表明，在土壤中添加硅（Si）对水稻植株的生理有各种有益的影响。本研究鉴定并克隆了水稻硅吸收相关基因low Si-influx 1（Lsi 1）。为了更好地了解硅处理、镉胁迫和水稻生理的相关机制，研究了转Lsi 1基因Lemont水稻在镉胁迫下的光合速率（Pn）、籽粒产量和对镉胁迫的抗性。施硅肥显著降低了镉胁迫下水稻体内镉的含量。在高Cd胁迫下，Lsi 1-OE转基因Lemont水稻的抗Cd效应最为显著。相反，土壤中的镉降低了植物的硅吸收，表明两种元素之间的显着交互作用。在灌浆期，镉胁迫使水稻叶绿素含量和净光合速率显著降低，导致水稻减产。然而，Lsi 1-OE系具有比WT或Lsi 1-RNAi转基因Lemont水稻（Lsi 1-RNAi系）更高的叶绿素含量和Pn，保持了高的光合同化物运输以获得高产潜力。在收获时，Lsi 1-OE系比WT含有更多的Si和更少的Cd，而Lsi 1-RNAi系显示出相反的结果。总体而言，镉胁迫降低了水稻的产量，而施硅肥显著提高了水稻的产量。而野生型与Lsi 1-RNAi株系的籽粒产量差异不显著。这可能是由于Lsi 1-RNAi株系产生的补偿效应。土壤中的Si、Lsi 1表达的增强或抑制以及植物对Cd毒害的抗性都能显著影响水稻产量，因此改变这些因素是提高产量的一种可行途径。
Many publications indicated various beneficial effects of the addition of silicon (Si) in soil on the physiology of rice plants. The gene responsible for the Si-uptake in rice, low Si-influx 1 (Lsi1), was identified and cloned for this study. The photosynthetic rate (Pn), grain yield, and resistance to Cadmium (Cd)-stress of the wild-type (WT) andLsi1-transgenic Lemont rice lines under Cd-stress were examined in an attempt to better understand the mechanism associated with the Si-addition, Cd-stress, and rice physiology. Si-fertilization significantly reduced the Cd-content in rice under Cd-stress. The effect was most significant in theLsi1-overexpression transgenic Lemont rice (Lsi1-OE line) under high Cd-stress. Conversely, Cd in soil lowered the Si-uptake of the plants indicating a significant interaction between the two elements. During the grain-filling period, Cd-stress greatly reduced the chlorophyll content and Pn of the rice resulting in a diminished grain output. However, Lsi1-OE line with a higher chlorophyll content and Pn than either WT orLsi1-RNAi transgenic Lemont rice (Lsi1-RNAi line) maintained a high photo-assimilate transportation for high yield potential. At harvest, Lsi1-OE line contained more Si and less Cd than WT, whereas the Lsi1-RNAi line showed an opposite result. In general, Cd-stress reduced, while Si-fertilization significantly increased, the grain yield on rice. However, no significant difference on the grain yields existed between WT and Lsi1-RNAi line. This might be due to a compensation effect generated by Lsi1-RNAi line. It appeared that Si in the soil, as well as the enhancing or inhibitingLsi1expression and the resistance to Cd-toxicity of the plants, could significantly affect the rice yield making alternations on these factors a plausible approach for production improvement.