Photoincorporation of 4,4'-bis(1-anilino-8-naphthalenesulfonic acid) into the apical domain of GroEL: specific information from a nonspecific probe.
Photoincorporation of 4,4'-bis(1-anilino-8-naphthalenesulfonic acid) into the apical domain of GroEL: specific information from a nonspecific probe.
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4,4-双（1-苯胺基-8-萘磺酸）光掺入 GroEL 的顶端结构域：来自非特异性探针的特异性信息。
DOI：
10.1021/bi00022a018
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Horowitz,PM
中科院分区：
文献类型：
--
作者：
Seale,JW;Martinez,JL;Horowitz,PM
In this report, we extend the usefulness of the probe bis-ANS by showing that it can be covalently photoincorporated into various proteins. Using the chaperonin GroEL, we have shown that it is possible to locate important hydrophobic surfaces throughphotoincorporation and peptide sequencing. It has been proposed that hydrophobic surfaces on the chaperonin may be responsible for the binding of unfolded polypeptides. We show here that photoincorporation of bis-ANS is able to locate a distinct hydrophobic surface on GroEL. Incorporation of the bis-ANS occurs within a 45 residue fragment of the monomer near the middle of the primary sequence. Interestingly, photoincorporation occurs within this fragment in bothtetradecamers and assembly-competent monomers. From the three-dimensional structure of GroEL, this region mapsto the apicaldomain (residues 191—376), which has been implicated in polypeptide binding [Fenton, WA, Kashi, Y., Furtak, K., & Horwich, AL (1994) Nature 371, 614—619], In addition, the fluorescent properties of the probe are retained including the excitation and emission maxima and the sensitivity to the polarity of its environment. These results suggest that photoincorporated bis-ANS may be a useful probe for protein structure and dynamics.Proteins belonging to the class termed chaperonins have been implicated in such diversefunctions as protein traf-ficking, protein assembly, and assisted protein folding (Gething & Sambrook, 1992; Hendrick & Hard, 1993). Studies of chaperonin action have focused on the Escherichia coli chaperonins GroES and GroEL (Georgopoulos & Ang,
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DOI：
10.1016/s0021-9258(18)98800-9
发表时间：
1991-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
通讯作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
单体胰岛素类似物的动力学：检验熔球假说。
影响因子：
2.9
作者：
Hua,QX;Ladbury,JE;Weiss,MA
通讯作者：
Weiss,MA
大肠杆菌 groES 形态发生蛋白的纯化和性质。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandrasekhar,GN;Tilly,K;Woolford,C;Hendrix,R;Georgopoulos,C
通讯作者：
Georgopoulos,C
groE（一种参与噬菌体组装的宿主蛋白）的纯化和特性。
影响因子：
5.6
作者：
R. Hendrix
通讯作者：
R. Hendrix
影响因子：
56.9
作者：
AZEM, A;KESSEL, M;GOLOUBINOFF, P
通讯作者：
GOLOUBINOFF, P