Plant-Derived Human Collagen Scaffolds for Skin Tissue Engineering
Plant-Derived Human Collagen Scaffolds for Skin Tissue Engineering
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DOI：
10.1089/ten.tea.2012.0338
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发表时间：
2013-07-01
影响因子：
4.1
通讯作者：
Powell, Heather M.
中科院分区：
文献类型：
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作者：
Willard, James J.;Drexler, Jason W.;Powell, Heather M.
组织工程支架通常使用从动物组织如牛皮中提取的蛋白质形成。与使用这些材料相关的风险包括超敏反应和病原体污染。人源性蛋白质降低了超敏反应的风险，但具有疾病传播的风险。使用植物材料工程化重组人蛋白的方法提供了这些材料的替代来源，而没有疾病传播的风险或关于变异性的担忧。为探讨植物源性人胶原蛋白（PDHC）在工程皮肤构建中的应用，采用静电纺丝法和冷冻干燥法将PDHC与牛皮胶原蛋白复合制成组织工程支架。这两种原材料很容易形成两种常见的支架类型，静电纺丝非织造支架和冻干海绵，具有相似的结构。然而，PDHC的处理时间明显较短。PDHC支架支持原代人细胞附着和增殖，其水平与牛材料相当或更高。与牛胶原相比，当活化的THP-1巨噬细胞暴露于PDHC电纺支架时，白细胞介素-1 β的产生显著降低。两种材料均促进了ES细胞的成熟和分化。这些数据表明，PDHC可能提供一种新的来源的组织工程的原材料与低风险的过敏反应或疾病传播。
Tissue engineering scaffolds are commonly formed using proteins extracted from animal tissues, such as bovine hide. Risks associated with the use of these materials include hypersensitivity and pathogenic contamination. Human-derived proteins lower the risk of hypersensitivity, but possess the risk of disease transmission. Methods engineering recombinant human proteins using plant material provide an alternate source of these materials without the risk of disease transmission or concerns regarding variability. To investigate the utility of plant-derived human collagen (PDHC) in the development of engineered skin (ES), PDHC and bovine hide collagen were formed into tissue engineering scaffolds using electrospinning or freeze-drying. Both raw materials were easily formed into two common scaffold types, electrospun nonwoven scaffolds and lyophilized sponges, with similar architectures. The processing time, however, was significantly lower with PDHC. PDHC scaffolds supported primary human cell attachment and proliferation at an equivalent or higher level than the bovine material. Interleukin-1 beta production was significantly lower when activated THP-1 macrophages where exposed to PDHC electrospun scaffolds compared to bovine collagen. Both materials promoted proper maturation and differentiation of ES. These data suggest that PDHC may provide a novel source of raw material for tissue engineering with low risk of allergic response or disease transmission.