Sex-linked period genes in the silkmoth, Antheraea pernyi:: Implications for circadian clock regulation and the evolution of sex chromosomes
Sex-linked period genes in the silkmoth, Antheraea pernyi:: Implications for circadian clock regulation and the evolution of sex chromosomes
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DOI：
10.1016/s0896-6273(00)81042-9
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发表时间：
1999-12-01
期刊：
影响因子：
16.2
通讯作者：
Reppert, SM
中科院分区：
文献类型：
--
作者：
Gotter, AL;Levine, JD;Reppert, SM
周期（per）基因的调节是昆虫生物钟功能的一个关键特征。在这里，我们展示了per在柞蚕（Antheraea pernyi）中是性别连锁的。前面描述的每个基因都在Z染色体上发现。蚕蛾在RNA和蛋白质水平上都没有剂量补偿。虽然早期的研究表明每个转录本存在振荡的内源性反义，但我们表明该转录本来自女性特异性W染色体上的一个位点。我们进一步证明了在W上存在一个编码截断蛋白的per同源物。雄性（ZZ）飞蛾的节律性表明，两个w连锁的per-like基因都不是时钟功能所必需的。在W上有重复的per等位基因的存在提供了对性染色体进化的洞察。
Regulation of the period (per) gene is a critical feature of circadian clock function in insects. Here, we show that per is sex-linked in the silkmoth, Antheraea pernyi. The previously described silkmoth per gene is found on the Z chromosome. Silkmoth per is not dosage compensated at either the RNA or the protein level. Although earlier studies showed the presence of an oscillating endogenous antisense per transcript, we show that this transcript comes from a locus on the female-specific W chromosome. We further demonstrate the presence of a homolog of per on W that encodes a truncated protein. Rhythmicity of male (ZZ) moths demonstrates that neither of the W-linked per-like genes is essential for clock function. The presence of a per allele with duplications on W provides insight into the evolution of the sex chromosomes.