RELATION BETWEEN VARIOUS PHOSPHOLIPASE ACTIONS ON HUMAN RED-CELL MEMBRANES AND INTERFACIAL PHOSPHOLIPID PRESSURE IN MONOLAYERS
RELATION BETWEEN VARIOUS PHOSPHOLIPASE ACTIONS ON HUMAN RED-CELL MEMBRANES AND INTERFACIAL PHOSPHOLIPID PRESSURE IN MONOLAYERS
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DOI：
10.1016/0005-2736(75)90045-0
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
VANDEENEN, LLM
中科院分区：
文献类型：
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作者：
DEMEL, RA;GEURTSVANKESSEL, WSM;VANDEENEN, LLM
纯化的磷脂酶的各种界面压力的单分子膜上的行动进行比较的红细胞膜上的行动。不能水解完整红细胞膜磷脂的磷脂酶，如蜡样芽孢杆菌的磷脂酶C，猪胰和响尾蛇的磷脂酶A_2，卷心菜的磷脂酶D，在低于31 dynes/cm的压力下才能水解磷脂单层，能水解完整红细胞膜磷脂的磷脂酶，来自魏氏梭菌的磷脂酶C、来自眼镜蛇和蜂毒的磷脂酶A_2和来自金黄色葡萄球菌的鞘磷脂酶在31达因/厘米以上的压力下能水解磷脂单层。它的结论是，在红细胞膜的外单层的脂质包装是与侧面压力之间的31和34.8达因/厘米。
The action of purified phospholipases on monomolecular films of various interfacial pressures is compared with the action on erythrocyte membranes. The phospholipases which cannot hydrolyse phospholipids of the intact erythrocyte membrane, phospholipase C fromBacillus cereus, phospholipase A2from pig pancreas andCrotalus adamanteusand phospholipase D from cabbage, can hydrolyse phospholipid monolayers at pressure below 31 dynes/cm only.The phospholipases which can hydrolyse phospholipids of the intact erythrocyte membrane, phospholipase C fromClostridium welchiiphospholipase A2fromNaja najaand bee venom and sphingomyelinase fromStaphylococcus aureus, can hydrolyse phospholipid monolayers at pressure above 31 dynes/cm. It is concluded that the lipid packing in the outer monolayer of the erythrocyte membrane is comparable with a lateral surface pressure between 31 and 34.8 dynes/cm.