The use of human amniotic fluid stem cells as an adjunct to promote pulmonary development in a rabbit model for congenital diaphragmatic hernia
The use of human amniotic fluid stem cells as an adjunct to promote pulmonary development in a rabbit model for congenital diaphragmatic hernia
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DOI：
10.1002/pd.4621
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发表时间：
2015-09-01
影响因子：
3
通讯作者：
Deprest, Jan
中科院分区：
文献类型：
--
作者：
DeKoninck, Philip;Toelen, Jaan;Deprest, Jan
本研究旨在评估气管内注射人羊水干细胞（hAFSC）对肺发育结合TO在兔CDH.MethodsIn时间交配妊娠的模型的潜在好处，没有一个左肺缺损，在第23天（期限= 31）。在第28天，将先前手术的胎儿分配至TO并注射70 L磷酸盐缓冲盐水（PBS）或1.0 × 10（6）个表达LacZ的c-Kit阳性hAFSC，或保持不动（CDH）。在第31天进行收获以获得其肺/体重比（LBWR）、气道和血管肺形态计量学、X-gal染色和Ki67和表面活性蛋白-B（SP-B）的免疫组化。额外注射hAFSC降低了MTBD，并导致腺泡内血管的肌肉化不显著降低。有没有炎症变化和LacZ阳性hAFSC分散在整个肺实质4days afterinjection.ConclusionHAFSC发挥额外的影响TO导致减少MTBD，周围的终末细支气管肺泡数的措施，没有毒性的迹象。(c)2015年约翰威利父子有限公司
ObjectiveThis study aimed to evaluate the potential benefit of intra-tracheal injection of human amniotic fluid stem cells (hAFSC) on pulmonary development combined with TO in a rabbit model for CDH.MethodsIn time-mated pregnant does a left diaphragmatic defect was created at d23 (term=31). At d28, previously operated fetuses were assigned to either TO and injection with 70L of phosphate buffered saline (PBS) or 1.0x10(6) c-Kit positive hAFSC expressing LacZ or were left untouched (CDH). Harvesting was done at d31 to obtain their lung-to-body weight ratio (LBWR), airway and vascular lung morphometry, X-gal staining and immunohistochemistry for Ki67 and surfactant protein-B (SP-B).ResultsCDH-induced pulmonary hypoplasia is countered by TO+PBS, this reverses LBWR, mean terminal bronchiole density (MTBD) and medial thickness to normal. The additional injection of hAFSC decreases MTBD and results in a non-significant decrease in muscularization of intra-acinary vessels. There were no inflammatory changes and LacZ positive hAFSC were dispersed throughout the lung parenchyma 4days after injection.ConclusionHAFSC exert an additional effect on TO leading to a decrease in MTBD, a measure of alveolar number surrounding the terminal bronchioles, without signs of toxicity. (c) 2015 John Wiley & Sons, Ltd.