Control of cell survival and proliferation of postnatal PSA-NCAM+ progenitors
Control of cell survival and proliferation of postnatal PSA-NCAM+ progenitors
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DOI：
10.1016/s1044-7431(02)00030-1
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发表时间：
2003-02-01
影响因子：
3.5
通讯作者：
Schumacher, M
中科院分区：
文献类型：
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作者：
Gago, N;Avellana-Adalid, V;Schumacher, M
在本工作中，我们研究了几种生长因子对表达多唾液酸型神经细胞黏附分子(PSA-NCAM)的新鲜分离的神经祖细胞存活和增殖的影响。取出生后第2天的大鼠前脑，采用分离方法获得细胞。我们发现：(1)胰岛素样生长因子-1(IGF-1)通过抑制细胞凋亡而发挥强大的存活作用，(2)表皮生长因子(EGF)强烈促进细胞增殖，(3)IGF-1与EGF联合应用促进细胞扩张，(4)碱性成纤维细胞生长因子(BFGF)仅表现出微弱的促有丝分裂作用，(5)血小板衍生生长因子-AA(PDGF-AA)对细胞存活和增殖无影响。这些结果表明，本研究中描述的出生后PSA-NCAM(+)前体细胞可能代表着胚胎EGF反应性神经前体细胞和已描述的PDGF反应的出生后PSA-NCAM(+)前体细胞之间的过渡阶段。对于这些“早期PSA-NCAM(+)祖细胞”来说，胰岛素样生长因子1和EGF似乎在控制细胞死亡和细胞增殖方面起着关键作用。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
In the present work, we studied the effects of several growth factors on survival and proliferation of freshly isolated neural progenitors expressing the polysialylated form of neural cell adhesion molecule (PSA-NCAM). Cells were obtained from postnatal day 2 rat forebrain, using isolation method. We found that (1) insulin-like growth factor I (IGF-1) exerts a powerful survival effect by inhibiting apoptotic cell death, (2) epidermal growth factor (EGF) strongly increases cell proliferation, (3) the combination of IGF-1 plus EGF promotes cellular expansion, (4) basic fibroblast growth factor displays only a weak mitogenic effect, and (5) platelet-derived growth factor-AA (PDGF-AA) has no effect on cell survival and proliferation. These results suggest that the postnatal PSA-NCAM(+) progenitors characterized in the present work may represent a transitional stage, between the embryonic EGF-responsive neural progenitors and the postnatal PSA-NCAM(+) progenitors already described that are PDGF-responsive. For these "early PSA-NCAM(+) progenitors," insulin-like growth factor 1 and EGF seem to play a pivotal role in the control of cell death and cell proliferation. (C) 2003 Elsevier Science (USA). All rights reserved.