Two-step purification of scutellarin from Erigeron breviscapus (vant.) Hand. Mazz. by high-speed counter-current chromatography
Two-step purification of scutellarin from Erigeron breviscapus (vant.) Hand. Mazz. by high-speed counter-current chromatography
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DOI：
10.1016/j.jchromb.2006.04.030
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发表时间：
2006-07-11
影响因子：
3
通讯作者：
Liu, Chun-zhao
中科院分区：
文献类型：
--
作者：
Gao, Min;Gu, Ming;Liu, Chun-zhao
采用高速逆流色谱法（HSCCC）从灯盏花（Erigeron breviscapus（vant.）手马兹灯盏花是一种治疗心脏病的著名中药。采用正己烷-乙酸乙酯-甲醇-乙酸-水（1：6：1.5：1：4，v/v/v/v/v）和乙酸乙酯-正丁醇-乙腈-0.1%HCl（5：2：5：10，v/v/v/v）两种溶剂系统进行两步纯化。收集的野黄芩苷级分的纯度为95.6%。本研究为野黄芩苷的纯化提供了一种新的替代方法。(c)2006 Elsevier B. V.保留所有权利。
Scutellarin, a flavone glycoside, popularly applied for the treatment of cardiopathy, has been purified in two-step purification by high-speed counter-current chromatography (HSCCC) from Erigeron breviscapus (vant.) Hand. Mazz. (Deng-zhan-hua in Chinese), a well-known traditional Chinese medicinal plant for heart disease. Two solvent systems, n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1:4, v/v/v/v/v) and ethyl acetate-n-butanol-acetonitrile-0.1% HCl (5:2:5:10, v/v/v/v) were used for the two-step purification. The purity of the collected fraction of scutellarin was 95.6%. This study supplies a new alternative method for purification of scutellarin. (c) 2006 Elsevier B.V. All rights reserved.