First Report of SHV-148-Type ESBL and CMY-42-Type AmpC β-Lactamase in Klebsiella pneumoniae Clinical Isolates in Tunisia
First Report of SHV-148-Type ESBL and CMY-42-Type AmpC β-Lactamase in Klebsiella pneumoniae Clinical Isolates in Tunisia
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DOI：
10.1089/mdr.2018.0073
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发表时间：
2018-06-21
影响因子：
2.6
通讯作者：
Chouchani, Chedly
中科院分区：
文献类型：
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作者：
Dziri, Olfa;Dziri, Raoudha;Chouchani, Chedly
产超广谱β-内酰胺酶的肠杆菌科是世界范围内的一个真实的问题。我们的目的是调查从突尼斯东南部一家医院收集的肺炎克雷伯菌临床分离株对抗生素耐药的分子机制。18株头孢菌素耐药克雷伯氏菌。2015年4月至2016年8月期间回收了肺炎。通过PCR和测序对抗菌素耐药编码基因进行分子表征。结果显示，在我们分离的菌株中存在几种Ambler A类内酰胺酶编码基因：bla（CTXM-15）（15），bla（SHV-28）（6），bla（SHV-1）（2），bla（SHV-148）（1），bla（SHV-61）（1），bla（SHV-76）（1），bla（SHV-186）（1），bla（TEM-1）（8）。在9个分离株中观察到bla（OXA-1）的关联。而在4株分离株中检测到C-内酰胺酶编码基因[bla（CMY-4）（2），bla（CMY-42）（1），bla（ACT-35）（1）]。K. pneumoniae分离株通过脉冲场凝胶电泳显示16个不相关的脉冲型，证明我们的分离株之间的高度多样性。我们的研究提供了新的流行病学信息，显示了巨大的多样性，β-内酰胺酶编码基因在我们的菌株。事实上，这是SHV-76、SHV-148和SHV-186在突尼斯的首次报告。这也是CMY-42和ACT-35产生K.肺炎在我国
Extended-spectrum beta-lactamase producing Enterobacteriaceae present a real problem worldwide. We aimed to investigate the molecular mechanisms of resistance to antibiotics among Klebsiella pneumoniae clinical isolates collected from a Hospital in the southeast of Tunisia. Eighteen cephalosporin-resistant K. pneumoniae were recovered between April 2015 and August 2016. Molecular characterization of antimicrobial resistance encoding genes was performed by PCR and sequencing. Results revealed several types of Ambler class A -lactamase encoding genes among our isolates: [bla(CTXM-15) (15), bla(SHV-28) (6), bla(SHV-1) (2), bla(SHV-148) (1), bla(SHV-61) (1), bla(SHV-76) (1), bla(SHV-186) (1), bla(TEM-1) (8)]. The association of bla(OXA-1) was observed in nine isolates. However, the class C -lactamase encoding genes were detected in four isolates [bla(CMY-4) (2), bla(CMY-42) (1), bla(ACT-35) (1)]. Molecular typing of K. pneumoniae isolates by pulsed-field gel electrophoresis showed 16 unrelated pulsotypes proving a high diversity among our isolates. Our study provides new epidemiological information showing a huge diversity of -lactamase encoding genes among our isolates. In fact, this is the first report of SHV-76, SHV-148, and SHV-186 in Tunisia. This is also the first report of CMY-42 and ACT-35 producing K. pneumoniae in our country.