Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis
Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis
复制标题
DOI：
10.1128/jcm.39.9.3303-3310.2001
复制
发表时间：
2001-09-01
影响因子：
9.4
通讯作者：
Ko, AI
中科院分区：
文献类型：
--
作者：
Flannery, B;Costa, D;Ko, AI
钩端螺旋体病是一种新出现的人畜共患病，具有世界性分布，迫切需要发展新的血清学诊断策略。我们评价了5种重组抗原在钩端螺旋体病血清学诊断中的诊断效用。从50名健康居民的高发病率地区的血清被用来确定96%特异性的临界值。经显微镜凝集试验证实的50例钩体病患者的配对血清中，免疫球蛋白G（IgG）而不是IgM与重组钩体蛋白反应。重组LipL32 IgG ELISA在钩端螺旋体病急性期（56%）和恢复期（94%）的敏感性最高。基于重组OmpL1，LipL41和Hsp58的ELISA在急性期的敏感性分别为16%，24%和18%，在恢复期分别为72%，44%和32%。与健康人血清相比，患者血清与重组LipL36无显著反应（P > 0.05）。重组LipL32 IgG ELISA在100名健康个体中表现出95%的特异性，在30名登革热患者、30名肝炎患者和16名最初被认为是钩端螺旋体病的患者中表现出90%至97%的特异性。在39个性病研究实验室测试阳性的个人和30莱姆病患者，13和23%的血清，分别与rLipL32抗原呈阳性反应。这些结果表明，rLipL32可能是一个有用的抗原，钩体病的血清学诊断。
There is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis.