STIMULATION OF S14 MESSENGER-RNA AND LIPOGENESIS IN BROWN FAT BY HYPOTHYROIDISM, COLD-EXPOSURE, AND CAFETERIA FEEDING - EVIDENCE SUPPORTING A GENERAL ROLE FOR S14 IN LIPOGENESIS AND LIPOGENESIS IN THE MAINTENANCE OF THERMOGENESIS
STIMULATION OF S14 MESSENGER-RNA AND LIPOGENESIS IN BROWN FAT BY HYPOTHYROIDISM, COLD-EXPOSURE, AND CAFETERIA FEEDING - EVIDENCE SUPPORTING A GENERAL ROLE FOR S14 IN LIPOGENESIS AND LIPOGENESIS IN THE MAINTENANCE OF THERMOGENESIS
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DOI：
10.1073/pnas.84.9.3070
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
OPPENHEIMER, JH
中科院分区：
文献类型：
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作者：
FREAKE, HC;OPPENHEIMER, JH
在肝脏中，甲状腺激素快速诱导S14 mRNA，其编码一种小的酸性蛋白。该序列仅在脂肪生成组织中大量表达，并被认为在脂肪代谢中具有某些功能。在甲状腺功能正常的大鼠中，我们测量到肩胛间棕色脂肪组织中S14 mRNA的水平比肝脏高20倍。此外，在肝脏或附睾脂肪中，甲状腺功能减退导致S14 mRNA下降80%，而在棕色脂肪中，该序列的水平进一步增加了3倍。在所有三种组织中，S14 mRNA的表达与脂肪生成相关，如通过3H2O掺入所评估的。慢性冷暴露或自助餐喂养的棕色脂肪的生理激活增加了该组织中S14 mRNA的浓度，并再次伴随着更大的脂肪酸合成速率。总体而言，在肝脏和白色和棕色脂肪组织中，S14 mRNA和脂肪生成具有良好的相关性，强烈提示S14蛋白与脂肪合成相关的功能。这些研究表明，S14蛋白和脂肪生成可能是重要的甲状腺激素诱导的和棕色脂肪组织产热，这些功能的刺激，在甲状腺激素诱导的产热减少的结果，在其他地方的甲减棕色脂肪。
In liver, thyroid hormone rapidly induces S14 mRNA, which encodes a small acidic protein. This sequence is abundantly expressed only in lipogenic tissues and its thought to have some function in fat metabolism. In the euthyroid rat, we measured 20-fold higher levels of S14 mRNA in interscapular brown adipose tissue than liver. Furthermore, whereas in liver or epididymal fat, hypothyroidism resulted in an 80% fall in S14 mRNA, in brown fat the level of this sequence increased a further 3-fold. In all three tissues, the expression of S14 mRNA correlated well with lipogenesis, as assessed by 3H2O incorporation. Physiological activation of brown fat by chronic cold exposure or cafeteria feeding increased the concentration of S14 mRNA in this tissue and again this was accompanied by a greater rate of fatty acid synthesis. Overall, in liver and white and brown adipose tissue, S14 mRNA and lipogenesis were well correlated and strongly suggest a function of the S14 protein related to fat synthesis. These studies suggest that the S14 protein and lipogenesis may be important for thyroid hormone-induced and brown adipose tissue thermogenesis and that stimulation of these functions in hypothroid brown fat is a consequence of decreased thyroid hormone-induced thermogenesis elsewhere.