SANDWICH ELISA FOR DETECTION OF PICOGRAM QUANTITIES OF INTERLEUKIN-8
SANDWICH ELISA FOR DETECTION OF PICOGRAM QUANTITIES OF INTERLEUKIN-8
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DOI：
10.3109/08820139109054928
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发表时间：
1991-01-01
影响因子：
2.8
通讯作者：
REMICK, DG
中科院分区：
文献类型：
--
作者：
DEFORGE, LE;REMICK, DG
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was developed for interleukin-8 (IL-8), a neutrophil chemoattractant and activator. A polyclonal antibody to recombinant human IL-8 was raised in rabbits, and the IgG was isolated from the antisera using a protein A column. Native and biotinylated forms of this antibody served as the capture antibody and developing for the ELISA, respectively, and avidin-conjugated horse radish peroxidase provided the means for enzymatic color development. The lower limit of sensitivity for the assay was found to be 84 +/- 20 pg/ml IL-8 (mean +/- SD for 10 determinations). An inter-assay variability of 15-29% and an intra-assay variability of 12% were observed. The assay was able to detect IL-8 when the samples were prepared in either normal saline, RPMI, or human plasma. The development of this rapid, sensitive assay should provide a means to more fully evaluate the role of this cytokine in diverse disease states.
