The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
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DOI：
10.1080/10934529.2011.609036
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发表时间：
2011-10
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影响因子：
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通讯作者：
J. B. Costa;D. Rossi;Elisangela A De Souza;D. Samios;F. Bregalda;Maria do Carmo Ruaro Peralba;S. H. Flôres;M. A. Z. Ayub
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文献类型：
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作者：
J. B. Costa;D. Rossi;Elisangela A De Souza;D. Samios;F. Bregalda;Maria do Carmo Ruaro Peralba;S. H. Flôres;M. A. Z. Ayub
本研究对克雷伯氏肺炎杆菌BLb 01利用生物柴油生产厂排放的剩余甘油进行了产氢研究。从大豆加工厂UASB反应器的富菌污泥中分离到一株肺炎克雷伯氏菌BLb 01。采用Plackett-Burman设计（P-B）和响应面分析法（RSM）确定了提高产氢率的最佳条件。最大产氢量为45.0 mol %，甘油降解率为98%，使用以下组成的优化培养基实现：30 g L−1甘油; 3 g L−1酵母提取物; 3 g L−1 K2 HPO 4; 1 g L−1 KH 2 PO 4;温度39°C和pH 9.0。这些结果显示了这种新菌株有效地将残留甘油转化为增值能源产品的能力。
In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett–Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L−1 glycerol; 3 g L−1 yeast ex tract 3 g L−1 K2HPO4; 1 g L−1 KH2PO4; temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.