Cloning and characterization of two Campoletis chlorideae ichnovirus vankyrin genes expressed in parasitized host Helicoverpa armigera.
Cloning and characterization of two Campoletis chlorideae ichnovirus vankyrin genes expressed in parasitized host Helicoverpa armigera.
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DOI：
10.1016/j.jinsphys.2007.03.015
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发表时间：
2007-07
影响因子：
2.2
通讯作者：
S. Tian;Ji-Hong Zhang;Chenzhu Wang
中科院分区：
文献类型：
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作者：
S. Tian;Ji-Hong Zhang;Chenzhu Wang
多脱氧核糖核酸病毒是寄生姬蜂（ichnoviruses，IV）和茧蜂（bracoviruses，BV）的共生体，与黄蜂卵一起沿着注入宿主。在宿主内，PDV基因表达，其产物的功能是改变鳞翅目宿主的生理学，并使内寄生虫发育。在本研究中，我们描述了两个Campoletis chlorideae ichnovirus（CcIV）病毒锚蛋白（vankyrin）基因及其转录。CcIV vankyrin基因具有锚蛋白重复结构域，类似于果蝇NF-κB转录因子抑制剂（IκB）仙人掌的抑制结构域。棉铃虫CcIV vankyrin基因在棉铃虫寄生过程中均有表达，在寄生后30 min（p. p.）我们的数据表明，CcIV vankyrin基因在寄生宿主的组织中差异表达，并且两者都主要在血细胞中表达。两个CcIV vankyrin基因表达的时间和空间变化表明，CcIV vankyrin基因可能通过抑制NF-κB信号通路，从而改变被寄生鳞翅目昆虫宿主的发育和免疫应答，参与了寄生蜂卵对宿主细胞免疫应答的早期保护。
Polydnaviruses, symbionts of parasitic ichneumonid (ichnoviruses, IVs) and braconid (bracoviruses, BVs), are injected into hosts along with wasp eggs. Within the host, PDV genes are expressed and their products function to alter lepidopteran host physiology and enable endoparasitoid development. In the present study, we describe two Campoletis chlorideae ichnovirus (CcIV) viral ankyrin (vankyrin) genes and their transcription. The CcIV vankyrin genes possess ankyrin repeat domains that resemble the inhibitory domains of the Drosophila melanogaster NF-κB transcription factor inhibitor (IκB) cactus. The expression of CcIV vankyrin genes could be detected in Helicoverpa armigera during the whole course of parasitization with two expression peaks, 30min post-parasitization (p.p.) and 2 days p.p. Our data indicate that the CcIV vankyrin genes are differentially expressed in the tissues of parasitized hosts and both are mainly expressed in hemocytes. The temporal and spatial variation in expression of the two CcIV vankyrin genes suggests that CcIV vankyrin genes could be involved in early protection of parasitoid eggs from host cellular immune response by suppressing NF-κB signaling cascades, thereby altering development and immune responses of parasitized lepidopteran hosts.