A molecular network for functional versatility of HECATE transcription factors
A molecular network for functional versatility of HECATE transcription factors
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DOI：
10.1111/tpj.13930
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发表时间：
2018-07-01
期刊：
影响因子：
7.2
通讯作者：
Lohmann, Jan U.
中科院分区：
文献类型：
--
作者：
Gaillochet, Christophe;Jamge, Suraj;Lohmann, Jan U.
在植物生命周期中，不同的信号输入不断整合，并根据细胞或发育背景参与特定的遗传程序。Hecate(HEC)碱性螺旋-环-螺旋转录因子在这一过程中扮演着重要的角色，具有多种功能，从光形态发生到控制茎分生组织的动态和雌蕊群的形成。然而，其功能多样性和特定HEC子程序的部署背后的分子机制仍然难以捉摸。为了解决这个问题，我们系统地确定了具有与HEC1相互作用的能力的蛋白质，HEC1是该家族中特征最好的成员，并将这些信息与我们的直接HEC1靶基因数据集整合在一起。由此得到的核心遗传模块与HEC1的特定发育功能一致，包括其在光信号、雌蕊群发育和生长素稳态中所描述的活动。重要的是，我们发现HEC基因也在开花时间的调控中发挥作用，并发现它们在雌蕊群发育中的作用可能涉及NGATHA1(NGA1)和NGA2基因的直接转录调控。NGA因子以前被证明有助于果实的发育，但我们的数据现在表明，它们也调节茎顶端分生组织中的干细胞动态平衡。综上所述，我们的结果描绘了一个支持HEC转录因子功能多样性的分子网络。我们的分析不仅使我们能够确定控制茎段干细胞活性的相关靶基因和迄今尚未被描述的HEC1的生物学功能，而且为进一步阐明与上下文相关的HEC活性的机制提供了丰富的资源。
During the plant life cycle, diverse signaling inputs are continuously integrated and engage specific genetic programs depending on the cellular or developmental context. Consistent with an important role in this process, HECATE (HEC) basic helix-loop-helix transcription factors display diverse functions, from photomorphogenesis to the control of shoot meristem dynamics and gynoecium patterning. However, the molecular mechanisms underlying their functional versatility and the deployment of specific HEC subprograms remain elusive. To address this issue, we systematically identified proteins with the capacity to interact with HEC1, the best-characterized member of the family, and integrated this information with our data set of direct HEC1 target genes. The resulting core genetic modules were consistent with specific developmental functions of HEC1, including its described activities in light signaling, gynoecium development and auxin homeostasis. Importantly, we found that HEC genes also play a role in the modulation of flowering time, and uncovered that their role in gynoecium development may involve the direct transcriptional regulation of NGATHA1 (NGA1) and NGA2 genes. NGA factors were previously shown to contribute to fruit development, but our data now show that they also modulate stem cell homeostasis in the shoot apical meristem. Taken together, our results delineate a molecular network underlying the functional versatility of HEC transcription factors. Our analyses have not only allowed us to identify relevant target genes controlling shoot stem cell activity and a so far undescribed biological function of HEC1, but also provide a rich resource for the mechanistic elucidation of further context-dependent HEC activities.