Identification and functional characterization of polo-like kinase 2 autoregulatory sites.
Identification and functional characterization of polo-like kinase 2 autoregulatory sites.
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Polo 样激酶 2 自动调节位点的鉴定和功能表征。
DOI：
10.1016/j.neuroscience.2011.11.003
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发表时间：
2012
期刊：
影响因子：
3.3
通讯作者：
Pak,DTS
中科院分区：
文献类型：
--
作者：
Rozeboom,AM;Pak,DTS
波罗家族激酶在细胞增殖和神经元突触可塑性中起重要作用。然而，这些激酶的翻译后调节尚未完全了解。在这里，我们确定了几个新的Plk2磷酸化位点刺激Plk2本身。通过定点突变，我们发现了三个额外的过度激活Plk2突变以及一系列的残基调节Plk2稳态表达水平。由于Plk 2在兴奋性突触强度的稳态负控制中的既定作用，这些磷酸化位点在此过程中Plk 2信号的快速激活、扩展和延长中可能发挥重要作用。
Polo family kinases play important roles in cellular proliferation as well as neuronal synaptic plasticity. However, the posttranslational regulation of these kinases is not fully understood. Here, we identified several novel Plk2 phosphorylation sites stimulated by Plk2 itself. By site-directed mutagenesis, we uncovered three additional hyperactivating Plk2 mutations as well as a series of residues regulating Plk2 steady-state expression level. Because of the established role of Plk2 in homeostatic negative control of excitatory synaptic strength, these phosphorylation sites could play an important role in the rapid activation, expansion, and prolongation of Plk2 signaling in this process.