Experimental salivary pellicles on the surface of orthodontic materials
Experimental salivary pellicles on the surface of orthodontic materials
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DOI：
10.1067/mod.2001.110583
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发表时间：
2001-01-01
影响因子：
3
通讯作者：
Yang, WS
中科院分区：
文献类型：
--
作者：
Lee, SJ;Kho, HS;Yang, WS
本研究的目的是确定正畸材料表面形成的唾液膜的组成，并进一步研究不锈钢托槽金属、弹性结扎环和托槽粘合树脂3种不同正畸材料表面形成的吸附唾液膜的组成是否存在质的差异。实验用这些材料在新鲜人腮腺或下颌骨-舌下唾液中孵育2小时形成实验膜。用十二烷基硫酸钠缓冲液提取外膜并冻干。然后用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法鉴定吸附的唾液成分。根据正畸材料的类型，发现膜组分的轮廓存在显着差异。支架金属上的膜组分几乎与弹性结扎环上的膜组分相同。唾液蛋白对粘接树脂的吸附表现出不同的特征。不同腺体唾液中相同唾液蛋白的表面结合亲和力也存在明显差异。这些结果可能是基于材料表面特定蛋白质的结合位点被下颌-舌下唾液分子（可能是粘蛋白）所覆盖。本研究的结果为正畸材料表面的初始细菌粘附提供了有价值的信息，也为开发具有增强表面性能的正畸材料提供了信息。
The purpose of this study was to define the composition of salivary pellicles that form on the surfaces of orthodontic materials and to further investigate whether qualitative differences exist between the composition of adsorbed salivary pellicles that form on 3 different orthodontic materials: stainless steel bracket metal, elastomeric ligature ring, and bracket bonding resin. Experimental pellicles were formed by incubating these materials in fresh human parotid or submandibular-sublingual saliva for 2 hours. Pellicles were extracted with sodium dodecyl sulfate buffer and lyophilized. They were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to identify the adsorbed salivary components. Remarkable differences in the profiles of pellicle components were found, dependent on the type of orthodontic materials. The pellicle components on the bracket metal were almost the same as those found on the elastomeric ligature ring. Salivary protein adsorption patterns to bonding resin showed different features. Distinct differences were also found between the surface-binding affinities of the same salivary proteins from different glandular salivas. These results may be explained on the basis that binding sites for specific proteins on the surfaces of the materials are covered by molecules of submandibular-sublingual saliva, probably mucins. The results of this study provide valuable information concerning initial bacterial adhesion to the surfaces of orthodontic materials, as well as information that could be used in the development of orthodontic materials with enhanced surface properties.