Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes
Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes
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DOI：
10.1016/j.anireprosci.2009.03.004
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Pabuccuoglu, Serhat
中科院分区：
文献类型：
--
作者：
Evecen, Mithat;Cirit, Uemuet;Pabuccuoglu, Serhat
与其他家畜不同，犬卵母细胞体外成熟（IVM）的成功率仍然有限。研究了犬的发情周期和卵巢运输温度对犬卵母细胞体外成熟的影响。供体母犬根据发情周期的阶段分为三组：卵泡期（发情前期或发情期）、黄体期（间情期）和乏情期。从39只成熟母犬中收集的每对卵巢中的一个卵巢在4 ℃的磷酸盐缓冲盐水（PBS）中运输，而另一个卵巢在37 ℃下运输。将从所有卵巢获得的总共1138个I级COCs分组，并在38.5 ℃下在5%CO2、5%O-2和90%N-2的潮湿气氛中在添加促卵泡激素（FSH）、促黄体生成激素（LH）、必需和非必需氨基酸的改良合成输卵管液（mSOF）中成熟72 h。用乙酰地衣红染色法检测卵母细胞的核成熟率。在37 ℃组中，卵泡期和黄体期的卵母细胞的成熟率（MI+MII）显著高于无排卵期的卵母细胞（P
Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The present study investigated the effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. The donor bitches were categorized into three groups based on stage of estrus cycle: follicular (proestrus or estrous), luteal (diestrus) and anestrus. One ovary of each pair collected from 39 mature bitches was transported in Phosphate Buffer Saline (PBS) at 4 degrees C while the other was transported at 37 degrees C. A total of 1138 Grade I COCs obtained from all ovaries were grouped and matured in modified synthetic oviduct fluid (mSOF) supplemented with follicle stimulating hormone (FSH), luteinizing hormone (LH), essential and non-essential amino acids at 38.5 degrees C in a humidified 5% CO2, 5% O-2, and 90% N-2 atmosphere for 72 h. The nuclear maturation rates were evaluated by aceto-orcein staining.Oocytes harvested from follicular and luteal ovaries have a significantly higher maturation rates (MI+MII) than the oocytes from anestrual ovaries in the 37 degrees C group (p