Pheromone Studies of the Two spotted Spider Mite. 1. Evidence of a Sex Pheromone

Pheromone Studies of the Two spotted Spider Mite. 1. Evidence of a Sex Pheromone
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二斑蜘蛛的信息素研究。

DOI:
10.1093/jee/64.2.355
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发表时间:
1971
期刊:
影响因子:
--
通讯作者:
S. Burdajewicz
S. Burdajewicz
中科院分区:
--
文献类型:
--
作者:
W. Cone;L. McDonough;J. C. Maitlen;S. Burdajewicz

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雄性二斑叶螨对静止的后爪螨有强烈的吸引力,并且会一直停留到成年雌性出现,即交配发生。在实验室生物测定中,雄性被 1.5 微升静止后肢动物的粗乙醚提取物所吸引,每毫升溶剂中含有 0.01 个后肢动物。男性吸引力在测试的浓度范围内变化。最强的吸引力出现在每毫升 1.0 次生当量时,随着浓度的升高和降低,吸引力逐渐减弱。测试是在放置在潮湿的脱脂棉上的分离的啤酒花叶盘上进行的。一团湿润的细磨聚乙烯吡咯烷酮粉末是几种测试的粗提取物底物中最好的。男性吸引力被测量为对处理过的地点的反应的百分比,并针对偶尔访问未经处理的检查地点进行校正。对于每个测试点 1.5 微升提取物,男性反应平均为 37.2%(范围 0–73.6%)。需要进一步完善生物测定程序以帮助鉴定引诱剂。
Male Tetranychus urticae Koch are attracted strongly to quiescent deutonymphs and will remain until emergence of the adult female, when mating occurs. In laboratory bioassays, males were attracted to 1.5 µliters of crude ether extract of quiescent deutonymphs containing 0.01 deutonymphs per ml of solvent. Male attraction varied over the range of concentrations tested. Strongest attraction occurred at 1.0 deutonymph equivalents per ml with decreased attraction at the higher and lower concentrations. Tests were conducted on detached hop leaf discs placed upon moist absorbent cotton. A clump of moistened finely ground polyvinylpyrrolidone powder served as the best of several tested substrates for the crude extract. Male attraction was measured as a percent of responses to treated sites and corrected for occasional visits to untreated check sites. Male response averaged 37.2% (range 0–73.6%) for 1.5 µliters of extract per test site. Further refinement in bioassay procedure s necessary to aid in the identification of the attractant.